![Immunohistochemistry (Paraffin-embedded sections) - Bcr antibody [EP535Y] (ab40779) image](#ab40779b.jpg)
![Immunocytochemistry/ Immunofluorescence - Bcr antibody [EP535Y] (ab40779) image](#ab40779c.jpg)
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Products:Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Overview
Product name
Anti-Bcr antibody [EP535Y]
See all Bcr products (8) ...
Description
Rabbit monoclonal [EP535Y] to Bcr
Tested applications
WB, IHC-P, ICC, Flow Cyt, IPmore details
Cross reactivity
Reacts with
Human
Does not react with
Mouse, Rat
Immunogen
Synthetic peptide corresponding to residues in the C-terminus of human Bcr.
Positive control
K562 cell lysate, human lymphoma, HeLa cells.
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EP535Y
Isotype
IgG
Research areas
Cancer >> Signal transduction >> Protein phosphorylation >> Serine/threonine kinases >> Other
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab40779 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10,000.Detects a band of approximately 150 kDa (predicted molecular weight: 143 kDa).
IHC-P: 1/50 - 1/100.
ICC
ICC: 1/100 - 1/250.ICC: 1/100 - 1/250.
Flow Cyt
Flow Cyt: 1/20Flow Cyt: 1/20
IP
IP: 1/60IP: 1/60
Target
Relevance
The breakpoint cluster region protein (Bcr) is best know to be involved in genomic translocation with fusion partner Abl (Cbr-Abl) causing chronic myelogenous leukemia (CML). This 160 kDa protein contains a serine/threonine kinase domain, an SH2 binding domain, a GTP/GDP exchange domain and a C-term domain which functions as a GTPase activating protein for p21rac and CDC42. Additionally, Bcr is involved in signal transduction and can down regulate Ras mediated cell signaling.
Cellular localization
Cytoplasmic
Alternative names
- ALL antibody
- BCR 1 antibody
- BCR/ABL FUSION GENE INCLUDED antibody
see all
Database links
- Entrez Gene: 613 Human
- Omim: 151410 Human
- SwissProt: P11274 Human
- Unigene: 517461 Human
Anti-Bcr antibody [EP535Y] images:
Immunohistochemistry (Paraffin-embedded sections) - Bcr antibody [EP535Y] (ab40779)
![Immunohistochemistry (Paraffin-embedded sections) - Bcr antibody [EP535Y] (ab40779)](/ps/datasheet/Images/40/ab40779/ab40779b.jpg)
ab40779, staining human lymphoma by immunohistochemistry, paraffin embedded tissue.
Immunocytochemistry/ Immunofluorescence - Bcr antibody [EP535Y] (ab40779)
![Immunocytochemistry/ Immunofluorescence - Bcr antibody [EP535Y] (ab40779)](/ps/datasheet/Images/40/ab40779/ab40779c.jpg)
ab40779, staining HeLa cells by immunofluorescence.
References for Anti-Bcr antibody [EP535Y] (ab40779)
ab40779 has not yet been referenced specifically in any publications.
Publishing research using ab40779? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"