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Products:Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Overview
Product name
Anti-Bcr antibody
See all Bcr products (8) ...
Description
Rabbit polyclonal to Bcr
Tested applications
IHC-P, ICC/IF, IPmore details
Cross reactivity
Reacts with
Human
Does not react with
Mouse, Rat
Immunogen
A synthetic peptide corresponding to residues in the C-term of human Bcr.
Positive control
Jurkat cell lysate, human lung carcinoma and HeLa cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, 0.87% Sodium chloride, 0.09% Tris glycine
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Signal transduction >> Protein phosphorylation >> Serine/threonine kinases >> Other
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab32523 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ICC/IF: 1/250 - 1/500.
IHC-P: 1/100 - 1/250.
IP: 1/40.
Is unsuitable for FACS or WB.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
The breakpoint cluster region protein (Bcr) is best know to be involved in genomic translocation with fusion partner Abl (Cbr-Abl) causing chronic myelogenous leukemia (CML). This 160 kDa protein contains a serine/threonine kinase domain, an SH2 binding domain, a GTP/GDP exchange domain and a C-term domain which functions as a GTPase activating protein for p21rac and CDC42. Additionally, Bcr is involved in signal transduction and can down regulate Ras mediated cell signaling.
Cellular localization
Cytoplasmic
Alternative names
- ALL antibody
- BCR 1 antibody
- BCR/ABL FUSION GENE INCLUDED antibody
see all
Database links
- Entrez Gene: 613 Human
- Omim: 151410 Human
- SwissProt: P11274 Human
- Unigene: 517461 Human
Anti-Bcr antibody images:
Immunohistochemistry (Paraffin-embedded sections) - Bcr antibody (ab32523)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using ab32523 at 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - Bcr antibody (ab32523)
Immunofluorescent staining of HeLa cells using ab32523 at 1/250 dilution.
References for Anti-Bcr antibody (ab32523)
ab32523 has not yet been referenced specifically in any publications.
Publishing research using ab32523? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"