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Immunohistochemistry (Paraffin-embedded sections) - Bcr antibody (ab32523)
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Immunocytochemistry/ Immunofluorescence - Bcr antibody (ab32523)
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Product Name
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Bcr antibody
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See all Bcr antibodies (8)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Bcr
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Immunogen
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A synthetic peptide corresponding to residues in the C-term of human Bcr.
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Reacts with
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Hu
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Does not react with
(species key)
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Ms, Rat
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Tested applications
(see key)
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ICC/IF, IHC-P, IP
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: 1/250 - 1/500.
IHC-P: 1/100 - 1/250.
IP: 1/40.
Is unsuitable for FACS or WB.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Jurkat cell lysate, human lung carcinoma and HeLa cells.
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Cellular localization
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Cytoplasmic
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Research areas
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Cancer >> Signal transduction >> Protein phosphorylation >> Serine/threonine kinases >> Other
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
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Relevance
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The breakpoint cluster region protein (Bcr) is best know to be involved in genomic translocation with fusion partner Abl (Cbr-Abl) causing chronic myelogenous leukemia (CML). This 160 kDa protein contains a serine/threonine kinase domain, an SH2 binding domain, a GTP/GDP exchange domain and a C-term domain which functions as a GTPase activating protein for p21rac and CDC42. Additionally, Bcr is involved in signal transduction and can down regulate Ras mediated cell signaling.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: 0.25% Sodium Azide
Constituents: 40% Glycerol, 0.5% BSA, 0.18M Sodium chloride, 50mM Tris glycine, 25mM Citric acid, 0.25mM EDTA. pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Bcr antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Bcr antibody images, references, products related to ab32523 and other tools.
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Bcr antibody images:
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 Immunohistochemistry (Paraffin-embedded sections) - Bcr antibody (ab32523)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using ab32523 at 1/100 dilution.
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Immunocytochemistry/ Immunofluorescence - Bcr antibody (ab32523)
Immunofluorescent staining of HeLa cells using ab32523 at 1/250 dilution.
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Search PubMed (MEDLINE) for references to Bcr
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Bcr antibody - more information
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Customer reviews (feedback) regarding Bcr antibody |
Customer FAQs regarding Bcr antibody |
Protocols for Bcr antibody |
Price and availability of products related to Bcr antibody |
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Bcr antibody - related products:
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Bcr antibody - other tools:
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Contact Abcam with a Technical Enquiry about Bcr antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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