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If your product does not perform as described on this datasheet, we will refund or replace your product...
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Western blot - Bcr antibody (ab51087)
All lanes : Anti-Bcr antibody (ab51087) at 1/500 dilution
Lane 1 : K562 cell extract
Lane 2 : K562 cell extract + blocking peptide
Predicted band size : 143 kDa
Observed band size : 159 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Bcr antibody(ab51087)
Ab51087 staining human colon. Staining is localized to the cytoplasm.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system (Dako PT Link), at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer, EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
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