Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Overview
Product name
Anti-Bcr antibody
See all Bcr products (8) ...
Description
Rabbit polyclonal to Bcr
Specificity
ab51087 is capable of detecting endogenous levels of total Bcr protein.
Tested applications
IHC-P, WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Non-phosphopeptide derived from human Bcr around the phosphorylation site of tyrosine 177 (P-F-YP-V-N)
Positive control
K562 cell extracts.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Signal transduction >> Protein phosphorylation >> Serine/threonine kinases >> Other
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab51087 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 4 µg/mlPerform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
WB: 1/500 - 1/1000.Detects a band of approximately 159 kDa (predicted molecular weight: 143 kDa).
ELISA: Use at an assay dependent dilution.
Target
Relevance
The breakpoint cluster region protein (Bcr) is best know to be involved in genomic translocation with fusion partner Abl (Cbr-Abl) causing chronic myelogenous leukemia (CML). This 160 kDa protein contains a serine/threonine kinase domain, an SH2 binding domain, a GTP/GDP exchange domain and a C-term domain which functions as a GTPase activating protein for p21rac and CDC42. Additionally, Bcr is involved in signal transduction and can down regulate Ras mediated cell signaling.
Cellular localization
Cytoplasmic
Alternative names
- ALL antibody
- BCR1 antibody
- Breakpoint cluster region antibody
see all
Database links
- Entrez Gene: 613 Human
- Entrez Gene: 110279 Mouse
- Entrez Gene: 309696 Rat
- Omim: 151410 Human
- SwissProt: P11274 Human
- SwissProt: Q61223 Mouse
- Unigene: 517461 Human
Anti-Bcr antibody images:
Western blot - Bcr antibody (ab51087)
All lanes : Anti-Bcr antibody (ab51087) at 1/500 dilution
Lane 1 : K562 cell extract
Lane 2 : K562 cell extract + blocking peptide
Predicted band size : 143 kDa
Observed band size : 159 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Bcr antibody(ab51087)
Ab51087 staining human colon. Staining is localized to the cytoplasm.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system (Dako PT Link), at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer, EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
References for Anti-Bcr antibody (ab51087)
ab51087 has not yet been referenced specifically in any publications.
Publishing research using ab51087? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"