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Anti-Bcr antibody (ab51087)

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Overview

Product name

Anti-Bcr antibody
See all Bcr products (8) ...

Description

Rabbit polyclonal to Bcr

Specificity

ab51087 is capable of detecting endogenous levels of total Bcr protein.

Tested applications

IHC-P, WB, ELISAmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat

Immunogen

Non-phosphopeptide derived from human Bcr around the phosphorylation site of tyrosine 177 (P-F-YP-V-N)

Positive control

K562 cell extracts.

Properties

Form

Liquid

Storage instructions

Store at -20°C. Stable for 12 months at -20°C

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

  • Western blot - Bcr antibody (ab51087)Western blot - Bcr antibody (ab51087) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Bcr antibody(ab51087)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Bcr antibody(ab51087) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab51087 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

The breakpoint cluster region protein (Bcr) is best know to be involved in genomic translocation with fusion partner Abl (Cbr-Abl) causing chronic myelogenous leukemia (CML). This 160 kDa protein contains a serine/threonine kinase domain, an SH2 binding domain, a GTP/GDP exchange domain and a C-term domain which functions as a GTPase activating protein for p21rac and CDC42. Additionally, Bcr is involved in signal transduction and can down regulate Ras mediated cell signaling.

Cellular localization

Cytoplasmic

Alternative names

  • ALL antibody
  • BCR1 antibody
  • Breakpoint cluster region antibody
  • CML antibody
  • PHL antibody
see all

Anti-Bcr antibody images:

  Western blot - Bcr antibody (ab51087)

Western blot - Bcr antibody (ab51087)

All lanes : Anti-Bcr antibody (ab51087) at 1/500 dilution

Lane 1 : K562 cell extract
Lane 2 : K562 cell extract + blocking peptide


Predicted band size : 143 kDa
Observed band size : 159 kDa (why is the actual band size different from the predicted?)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Bcr antibody(ab51087)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Bcr antibody(ab51087)

Ab51087 staining human colon. Staining is localized to the cytoplasm.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system (Dako PT Link), at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer, EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

References for Anti-Bcr antibody (ab51087)

ab51087 has not yet been referenced specifically in any publications.

Publishing research using ab51087? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"