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Western blot - Bcr antibody (ab51087)
Western blot - Bcr antibody (ab51087)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Bcr antibody(ab51087)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Bcr antibody(ab51087)
(enlarge)


Product Name 

Bcr antibody

 

See all Bcr antibodies (8)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Bcr

Immunogen 

Non-phosphopeptide derived from human Bcr around the phosphorylation site of tyrosine 177 (P-F-YP-V-N)

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

ab51087 is capable of detecting endogenous levels of total Bcr protein.

Tested applications 
(see key)

ELISA, IHC-P, WB


Abreviews 

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(see key)


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Application notes 
(see key)

Recommended dilutions
ELISA: 1/20000.
WB: 1/500 - 1/1000. Detects a band of approximately 159 kDa (predicted molecular weight: 143 kDa).
IHC-P: Use at a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

K562 cell extracts.

Cellular localization 

Cytoplasmic

Research areas 

Cancer >> Signal transduction >> Protein phosphorylation >> Serine/threonine kinases >> Other
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases

Relevance 

The breakpoint cluster region protein (Bcr) is best know to be involved in genomic translocation with fusion partner Abl (Cbr-Abl) causing chronic myelogenous leukemia (CML). This 160 kDa protein contains a serine/threonine kinase domain, an SH2 binding domain, a GTP/GDP exchange domain and a C-term domain which functions as a GTPase activating protein for p21rac and CDC42. Additionally, Bcr is involved in signal transduction and can down regulate Ras mediated cell signaling.

 

Alternative names Bcr antibody (ab51087)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

613    (Human)
110279    (Mouse)
309696    (Rat)

Omim

    

151410    (Human)

SwissProt

    

P11274    (Human)
Q61223    (Mouse)

Unigene

    

517461    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at -20°C. Stable for 12 months at -20°C

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Bcr antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for Bcr antibody images, references, products related to ab51087 and other tools.

 

Bcr antibody images:

Product image  Western blot - Bcr antibody (ab51087)

Western blot - Bcr antibody (ab51087)

All lanes : Bcr antibody (ab51087) at 1/500 dilution

Lane 1 : K562 cell extract
Lane 2 : K562 cell extract + blocking peptide


Predicted band size : 143 kDa
Observed band size : 159 kDa (why is the actual band size different from the predicted?)

 

Product image  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Bcr antibody(ab51087)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Bcr antibody(ab51087)

Ab51087 staining human colon. Staining is localized to the cytoplasm.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system (Dako PT Link), at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer, EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

 
 

Search PubMed (MEDLINE) for references to Bcr

 

Bcr antibody - more information

Customer reviews (feedback) regarding Bcr antibody

Customer FAQs regarding Bcr antibody

Protocols for Bcr antibody

Price and availability of products related to Bcr antibody

 

Bcr antibody - related products:

Products for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Slides

Products for Western blot

Loading controls

Lysates

 

Corresponding phospho antibody for Bcr antibody

ab51040

  

Rabbit polyclonal to Bcr (phospho Y177)

 

Positive Controls for Bcr antibody

ab29307

  

K562 (Human erythromyeloblastoid leukemia cell line) Cytoplasmic Lysate - tumor cell line

 

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Other Bcr Antibodies

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Bcr antibody - other tools:

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