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If your product does not perform as described on this datasheet, we will refund or replace your product...
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Western blot - Bcr antibody (ab86173)
All lanes : Anti-Bcr antibody (ab86173) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
developed using the ECL technique
Predicted band size : 143 kDa
Exposure time : 30 seconds
Immunoprecipitation - Bcr antibody (ab86173)
Detection of Human Bcr by Immunoprecipitation, using ab86173 at 10µg/mg lysate. Image shows immunoprecipitated Bcr detected with post IP WB, loading 20% of IP and using HeLa whole cell lysate at 1mg, with an antibody recognising an upstream epitope in lane 1, ab86173 at 1 µg/ml in lane 2 and control IgG in lane 3. Detection: Chemiluminescence with an exposure time of 10 seconds.
Flow Cytometry - Bcr antibody (ab86173)
Flow Cytometric Detection of Bcr. 1 x 106 K562 cells were fixed, permeabilized, and stained with ab86173 at 0.25 µg in a 150 µl reaction. Image shows Isotype anti-KLH control (red), no antibody (blue) and ab86173 (black).
Immunocytochemistry/ Immunofluorescence - Anti-Bcr antibody (ab86173)
ICC/IF image of ab86173 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab86173, 5µg/ml) overnight at +4ºC. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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