Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Overview
Product name
Anti-Bcr (phospho Y177) antibody
See all Bcr products (8) ...
Description
Rabbit polyclonal to Bcr (phospho Y177)
Specificity
The antibody detects endogenous levels of Bcr only when phosphorylated at tyrosine 177
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Rat
Immunogen
Synthetic phosphopeptide derived from human Bcr around the phosphorylation site of tyrosine 177 (P-F-YP-V-N).
Positive control
Extracts form K562 cells
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Signal transduction >> Protein phosphorylation >> Serine/threonine kinases >> Other
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
-
Western blot - Bcr (phospho Y177) antibody (ab51040)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab51040 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/10000ELISA: 1/10000
WB: 1/500 - 1/1000.Detects a band of approximately 143 kDa (predicted molecular weight: 143 kDa).
Target
Relevance
The breakpoint cluster region protein (Bcr) is best know to be involved in genomic translocation with fusion partner Abl (Cbr-Abl) causing chronic myelogenous leukemia (CML). This 160 kDa protein contains a serine/threonine kinase domain, an SH2 binding domain, a GTP/GDP exchange domain and a C-term domain which functions as a GTPase activating protein for p21rac and CDC42. Additionally, Bcr is involved in signal transduction and can down regulate Ras mediated cell signaling.
Cellular localization
Cytoplasmic
Alternative names
- ALL antibody
- BCR 1 antibody
- BCR/ABL FUSION GENE INCLUDED antibody
see all
Database links
- Entrez Gene: 613 Human
- Entrez Gene: 309696 Rat
- Omim: 151410 Human
- SwissProt: P11274 Human
- Unigene: 517461 Human
Anti-Bcr (phospho Y177) antibody images:
Western blot - Bcr (phospho Y177) antibody (ab51040)
All lanes : Anti-Bcr (phospho Y177) antibody (ab51040) at 1/500 dilution
Lane 1 : K562 cell extract, treated with the immunising peptide.
Lane 2 : K562 cell extract, untreated.
Predicted band size : 143 kDa
Observed band size : 143 kDa
References for Anti-Bcr (phospho Y177) antibody (ab51040)
ab51040 has not yet been referenced specifically in any publications.
Publishing research using ab51040? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"