Anti-Beclin 1 antibody [2A4] (ab114071)
Key features and details
- Mouse monoclonal [2A4] to Beclin 1
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Beclin 1 antibody [2A4]
See all Beclin 1 primary antibodies -
Description
Mouse monoclonal [2A4] to Beclin 1 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Beclin 1 aa 51-248.
Database link: Q14457 -
Positive control
- WB: Hela; A431; MCF7; RAJI; Jurkat and SKBR3 cell lysates IHC-P: Breast cancer and liver cancer tissues
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
2A4 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab114071 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/500 - 1/2000. Predicted molecular weight: 60 kDa.
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IHC-P |
1/200 - 1/1000.
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Flow Cyt |
1/200 - 1/400.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 60 kDa. |
IHC-P
1/200 - 1/1000. |
Flow Cyt
1/200 - 1/400. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Plays a central role in autophagy (By similarity). May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the beclin family. -
Cellular localization
Golgi apparatus > trans-Golgi network membrane. Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells. - Information by UniProt
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Database links
- Entrez Gene: 8678 Human
- Omim: 604378 Human
- SwissProt: Q14457 Human
- Unigene: 716464 Human
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Alternative names
- APG6 antibody
- ATG 6 antibody
- ATG6 antibody
see all
Images
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All lanes : Anti-Beclin 1 antibody [2A4] (ab114071) at 1/500 dilution
Lane 1 : Hela cell lysate
Lane 2 : A431 cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : Raji cell lysate
Lane 5 : Jurkat cell lysate
Lane 6 : SKBR3 cell lysate
Predicted band size: 60 kDa -
Immunohistochemical analysis of Beclin 1 expression in paraffin embedded breast cancer tissues (left) and liver cancer tissues (right) using ab114071 at 1/200 dilution with DAB staining
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Flow cytometric analysis of RAJI cells using Beclin 1 mouse mAb (green) and negative control (purple).
Protocols
Datasheets and documents
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Datasheet download
References (28)
ab114071 has been referenced in 28 publications.
- Wu MD et al. Acetylshikonin induces autophagy-dependent apoptosis through the key LKB1-AMPK and PI3K/Akt-regulated mTOR signalling pathways in HL-60 cells. J Cell Mol Med 26:1606-1620 (2022). PubMed: 35106915
- Zhang Y et al. TP53 loss-of-function causes vulnerability to autophagy inhibition in aggressive prostate cancer. Int J Urol 29:1085-1094 (2022). PubMed: 35975690
- Zhou Y et al. MicroRNA-106a-5p promotes the proliferation, autophagy and migration of lung adenocarcinoma cells by targeting LKB1/AMPK. Exp Ther Med 22:1422 (2021). PubMed: 34707704
- Zhu Y et al. SOX2 promotes chemoresistance, cancer stem cells properties, and epithelial-mesenchymal transition by ß-catenin and Beclin1/autophagy signaling in colorectal cancer. Cell Death Dis 12:449 (2021). PubMed: 33953166
- Wang Y et al. Prognostic Value of Autophagy, Microsatellite Instability, and KRAS Mutations in Colorectal Cancer. J Cancer 12:3515-3528 (2021). PubMed: 33995628