Recombinant Anti-Beclin 1 antibody [EPR19662] - BSA and Azide free (ab227107)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19662] to Beclin 1 - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Beclin 1 antibody [EPR19662] - BSA and Azide free
See all Beclin 1 primary antibodies -
Description
Rabbit monoclonal [EPR19662] to Beclin 1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal brain, fetal heart and fetal kidney lysates; HeLa, HepG2, 293, MCF7, Jurkat, Raji, C6, RAW264.7, PC-12, NIH/3T3, A20 and C2C12 whole cell lysates; rat brain, kidney and spleen lysates; mouse brain, heart and spleen lysates. IP: HeLa whole cell lysate.
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General notes
ab227107 is the carrier-free version of ab207612.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19662 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227107 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
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IP |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). |
IP
Use at an assay dependent concentration. |
Target
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Function
Plays a central role in autophagy (By similarity). May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the beclin family. -
Cellular localization
Golgi apparatus > trans-Golgi network membrane. Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells. - Information by UniProt
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Database links
- Entrez Gene: 8678 Human
- Entrez Gene: 56208 Mouse
- Entrez Gene: 114558 Rat
- Omim: 604378 Human
- SwissProt: Q14457 Human
- SwissProt: O88597 Mouse
- SwissProt: Q91XJ1 Rat
- Unigene: 716464 Human
see all -
Alternative names
- APG6 antibody
- ATG 6 antibody
- ATG6 antibody
see all
Images
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Beclin 1 was immunoprecipitated from 0.35 mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab207612 at 1/30 dilution.
Western blot was performed from the immunoprecipitate using ab207612 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: HeLa whole cell lysate 10µg (Input).
Lane 2: ab207612 IP in HeLa whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab207612 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207612).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (3)
ab227107 has been referenced in 3 publications.
- Hu Q et al. Blockade of GCH1/BH4 Axis Activates Ferritinophagy to Mitigate the Resistance of Colorectal Cancer to Erastin-Induced Ferroptosis. Front Cell Dev Biol 10:810327 (2022). PubMed: 35223839
- Zhang Z et al. RNA-binding protein ZFP36/TTP protects against ferroptosis by regulating autophagy signaling pathway in hepatic stellate cells. Autophagy 16:1482-1505 (2020). PubMed: 31679460
- Wang X et al. Role of hypoxia-inducible factor-1a in autophagic cell death in microglial cells induced by hypoxia. Mol Med Rep 15:2097-2105 (2017). PubMed: 28259912