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Product name
Anti-Beclin 1 antibody
See all Beclin 1 products (16) ...
Description
Rabbit polyclonal to Beclin 1
Tested applications
ICC/IF, WB, IP, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat
Immunogen
Synthetic peptide (Human) (Internal sequence within residues 1-100).
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: Tris buffered saline, 5mg/ml BSA
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Pathways and Processes >> Metabolism processes >> Autophagy and mitophagy
Cancer >> Signal transduction >> Autophagy
Microbiology >> Interspecies Interaction >> Host Immune Response
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Tumor Suppressors
Applications
Show applications key
Our Abpromise guarantee covers the use of ab16998 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: 1/50 - 1/200.
WB: Use at an assay dependent dilution. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).1/100 (lot A), 1/2000-1/5000 (current lot).This datasheet has changed from the Lot A datasheet (sold out). Please note the concentration and suggested dilution changes.
IP: 1/80. (lot A).
IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Target
Function
Plays a central role in autophagy (By similarity). May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.
Tissue specificity
Ubiquitous.
Sequence similarities
Belongs to the beclin family.
Cellular localization
Golgi apparatus > trans-Golgi network membrane. Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells.
Target information above from: UniProt accessionQ14457
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- APG6 antibody
- ATG 6 antibody
- ATG6 antibody
see all
Database links
- Entrez Gene: 8678 Human
- Entrez Gene: 56208 Mouse
- Entrez Gene: 114558 Rat
- Omim: 604378 Human
- SwissProt: Q14457 Human
- SwissProt: O88597 Mouse
- SwissProt: Q91XJ1 Rat
- Unigene: 716464 Human
see all
Anti-Beclin 1 antibody images:
Western blot - Beclin 1 antibody (ab16998)
Anti-Beclin 1 antibody (ab16998) + Mouse liver tissue lysate at 50 µg
Predicted band size : 52 kDa
Observed band size : 52 kDa
ECL detection at 1 minute.
Immunocytochemistry/ Immunofluorescence-Beclin 1 antibody(ab16998)
ICC/IF image of ab16998 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16998, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Beclin 1 antibody (ab16998)
This product has been referenced in:
- Rami A et al. Focal cerebral ischemia induces upregulation of Beclin 1 and autophagy-like cell death. Neurobiol Dis 29:132-41 (2008).Read more (PubMed: 17936001) »
- Massey AC et al. Consequences of the selective blockage of chaperone-mediated autophagy. Proc Natl Acad Sci U S A 103:5805-10 (2006).Read more (PubMed: 16585521) »
See all 2 publications for this product
Publishing research using ab16998? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"