Anti-Beclin 1 antibody (ab217179)
Key features and details
- Rabbit polyclonal to Beclin 1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Beclin 1 antibody
See all Beclin 1 primary antibodies -
Description
Rabbit polyclonal to Beclin 1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Beclin 1 aa 200-300 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q14457 -
Positive control
- Mouse intestine lysates; rat kidney, rat brain and rat stomach tissues; 293T lysates; U138 and SMMC7721 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X
Aqueous buffered solution -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab217179 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/100 - 1/1000. Predicted molecular weight: 52 kDa.
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IHC-P |
1/100 - 1/500.
When using a fluorescent probe the recommended dilution is 1/50 – 1/200. |
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ICC/IF |
1/50 - 1/200.
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Notes |
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WB
1/100 - 1/1000. Predicted molecular weight: 52 kDa. |
IHC-P
1/100 - 1/500. When using a fluorescent probe the recommended dilution is 1/50 – 1/200. |
ICC/IF
1/50 - 1/200. |
Target
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Function
Plays a central role in autophagy (By similarity). May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the beclin family. -
Cellular localization
Golgi apparatus > trans-Golgi network membrane. Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells. - Information by UniProt
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Database links
- Entrez Gene: 8678 Human
- Entrez Gene: 56208 Mouse
- Entrez Gene: 114558 Rat
- Omim: 604378 Human
- SwissProt: Q14457 Human
- SwissProt: O88597 Mouse
- SwissProt: Q91XJ1 Rat
- Unigene: 716464 Human
see all -
Alternative names
- APG6 antibody
- ATG 6 antibody
- ATG6 antibody
see all
Images
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Anti-Beclin 1 antibody (ab217179) at 1/300 dilution + 293T lysate
Secondary
Goat Anti-Rabbit IgG Antibody (H+L), HRP at 1/5000 dilution
Predicted band size: 52 kDa -
Immunofluorescent analysis of SMMC7721 cells labeling Beclin 1 with ab217179 at 1/50 dilution followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
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All lanes : Anti-Beclin 1 antibody (ab217179) at 1/200 dilution
Lane 1 : mouse brain lysates
Lane 2 : mouse intestine lysates
Secondary
All lanes : Goat Anti-Rabbit IgG Antibody (H+L), HRP at 1/3000 dilution
Predicted band size: 52 kDa -
Immunofluorescent analysis of U138 cells labeling Beclin 1 with ab217179 at 1/100 dilution followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
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Immunohistochemical analysis of formalin-fixed and paraffin-embedded rat stomach tissue labeling Beclin 1 with ab217179 at 1/200 dilution, followed by conjugation to the secondary antibody (Goat Anti-Rabbit IgG, Cy3 conjugated) at 1/200 dilution.
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Immunohistochemical analysis of formalin-fixed and paraffin-embedded rat brain tissue labeling Beclin 1 with ab217179 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
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Immunohistochemical analysis of formalin-fixed and paraffin-embedded rat kidney tissue labeling Beclin 1 with ab217179 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab217179 has been referenced in 11 publications.
- Wang M et al. Neuroprotective Mechanism of Icariin on Hypoxic Ischemic Brain Damage in Neonatal Mice. Oxid Med Cell Longev 2022:1330928 (2022). PubMed: 36425058
- Zhu S et al. AAV2-mediated and hypoxia response element-directed expression of bFGF in neural stem cells showed therapeutic effects on spinal cord injury in rats. Cell Death Dis 12:274 (2021). PubMed: 33723238
- Dong Z et al. Inhibition of the Wnt/ß-catenin signaling pathway reduces autophagy levels in complement treated podocytes. Exp Ther Med 22:737 (2021). PubMed: 34055054
- Suknovic N et al. The ULK1 kinase, a necessary component of the pro-regenerative and anti-aging machinery in Hydra. Mech Ageing Dev 194:111414 (2021). PubMed: 33338499
- Fu H et al. Reticulocalbin 1 is required for proliferation and migration of non-small cell lung cancer cells regulated by osteoblast-conditioned medium. J Cell Mol Med 25:11198-11211 (2021). PubMed: 34747128