Overview

  • Product name
  • Description
    Rabbit polyclonal to Beclin 1
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Beclin 1 (C terminal). From the C-terminus.
    Database link: Q14457

  • Positive control
    • WB: HEK-293T and HeLa cell lysates. ICC/IF: SK-N-BE cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab223372 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 52 kDa).
ICC/IF 1/100.

Target

  • Function
    Plays a central role in autophagy (By similarity). May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the beclin family.
  • Cellular localization
    Golgi apparatus > trans-Golgi network membrane. Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • APG6 antibody
    • ATG 6 antibody
    • ATG6 antibody
    • ATG6 autophagy related 6 homolog antibody
    • Bcl-2-interacting protein beclin antibody
    • Beclin 1 (coiled coil moesin like BCL2 interacting protein) antibody
    • Beclin 1 autophagy related antibody
    • Beclin-1 antibody
    • Beclin1 antibody
    • BECN 1 antibody
    • Becn1 antibody
    • BECN1_HUMAN antibody
    • Coiled coil myosin like BCL2 interacting protein antibody
    • Coiled-coil myosin-like BCL2-interacting protein antibody
    • GT197 antibody
    • Protein GT197 antibody
    • VPS 30 antibody
    • VPS30 antibody
    see all

Images

  • 4% formaldehyde-fixed SK-N-BE cells stained for Beclin 1 (green) using ab223372 at 1/100 dilution in ICC/IF. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1/100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain (red) at 1/1000 for 60 minutes at room temperature; DAPI (blue) nuclear stain at 1/5000 for 5 minutes at room temperature.

    (A) DAPI nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223372 (D) Merge.

  • Anti-Beclin 1 antibody (ab223372) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit HRP at 1/2000 dilution

    Predicted band size : 52 kDa
    Observed band size : 51 kDa (why is the actual band size different from the predicted?)


    Exposure time : 12 minutes

    Blocking buffer: 5% milk in TBST.

    Color Development: TMB solution.

    Primary and secondary antibodies were incubated for 1 hour at room temperature.

  • Anti-Beclin 1 antibody (ab223372) at 1/1000 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit HRP at 1/2000 dilution

    Predicted band size : 52 kDa
    Observed band size : 51 kDa (why is the actual band size different from the predicted?)


    Exposure time : 12 minutes

    Blocking buffer: 5% milk in TBST.

    Color Development: TMB solution.

    Primary and secondary antibodies were incubated for 1 hour at room temperature.

References

ab223372 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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