Anti-Beclin 1 antibody (ab62557)
Key features and details
- Rabbit polyclonal to Beclin 1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Beclin 1 antibody
See all Beclin 1 primary antibodies -
Description
Rabbit polyclonal to Beclin 1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human Beclin 1 (N terminal). ab62557 was raised against a 17 amino acid synthetic peptide from near the amino terminus of human Beclin-1. The immunogen is located within amino acids 40 - 90 of Beclin-1.
Database link: Q14457 -
Positive control
- WB: HEK-293, A549, HeLa, HepG2, K562, MCF7 and SK-N-SH cell lines. Mouse heart, brain and colon tissue. Rat liver, lung, brain and colon tissue. IHC-P: Human brain tissue, mouse kidney and rat liver and spleen tissues. IF: Mouse brain. ICC/IF: HeLa cells.
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General notes
For Western blot protocol see the applications section.
For information on sample preparation for western blot, please refer here.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab62557 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (2) |
Use a concentration of 2 µg/ml.
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WB | (4) |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
After transfer, wash membrane in TBST. Incubate membrane with 5% non-fat dry milk in TBS, 4C, overnight.. Dilute ab62557 to recommended dilution in 10mL of blocking buffer. Incubate membrane with ab62557 for 1 h at room temperature. Wash 3 times x 10 min in washing buffer with gentle agitation. Dilute 1μL secondary in 10mL of blocking buffer to make a 1:10,000 dilution (can vary from 1:2,000 to 1:10,000). Incubate membrane for 1 h at rt. Wash 3 x 10 min in washing buffer with gentle agitation. Running Buffer: 25mM Tris, 192 mM Glycine, 0.1% SDS. Transfer Buffer: 20mM Tris, 150mM Glycine, 20% Methanol, 0.038% SDS. Blocking Buffer: 5% non-fat dry milk, TBS. Wash Buffer (TBST): |
IF |
Use a concentration of 20 µg/ml.
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ICC/IF | (1) |
1/100.
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Notes |
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IHC-P
Use a concentration of 2 µg/ml. |
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). After transfer, wash membrane in TBST. Incubate membrane with 5% non-fat dry milk in TBS, 4C, overnight.. Dilute ab62557 to recommended dilution in 10mL of blocking buffer. Incubate membrane with ab62557 for 1 h at room temperature. Wash 3 times x 10 min in washing buffer with gentle agitation. Dilute 1μL secondary in 10mL of blocking buffer to make a 1:10,000 dilution (can vary from 1:2,000 to 1:10,000). Incubate membrane for 1 h at rt. Wash 3 x 10 min in washing buffer with gentle agitation. Running Buffer: 25mM Tris, 192 mM Glycine, 0.1% SDS. Transfer Buffer: 20mM Tris, 150mM Glycine, 20% Methanol, 0.038% SDS. Blocking Buffer: 5% non-fat dry milk, TBS. Wash Buffer (TBST): |
IF
Use a concentration of 20 µg/ml. |
ICC/IF
1/100. |
Target
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Function
Plays a central role in autophagy (By similarity). May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the beclin family. -
Cellular localization
Golgi apparatus > trans-Golgi network membrane. Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells. - Information by UniProt
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Database links
- Entrez Gene: 8678 Human
- Entrez Gene: 56208 Mouse
- Entrez Gene: 114558 Rat
- Omim: 604378 Human
- SwissProt: Q14457 Human
- SwissProt: O88597 Mouse
- SwissProt: Q91XJ1 Rat
- Unigene: 716464 Human
see all -
Alternative names
- APG6 antibody
- ATG 6 antibody
- ATG6 antibody
see all
Images
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All lanes : Anti-Beclin 1 antibody (ab62557) at 1 µg/ml
Lane 1 : 293 human cell line
Lane 2 : A549 human cell line
Lane 3 : HeLa cell line
Lane 4 : HepG2 cell line
Lane 5 : K562 cell line
Lane 6 : MCF7 cell line
Lane 7 : SK-N-SH cell line
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Rabbit IgG antibody (HRP) at 1/1000 dilution
Predicted band size: 52 kDaGel: 10%
Running condition: 130V, 1.5hrs
Transfer condition: Wet, 250mA, 2 hrs (NC membrane)
Blocking condition: 5% non-fat milk in TBS at 4 overnight
Primary antibody incubation: RT, 1hr
Secondary antibody incubation: RT, 1hr
Washing condition: 5 ml TBST, 4 x 5min
Exposure system: GE ECL 10 sec
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue labelling Beclin 1 with ab6257 at 1 µg/mL. Heat mediated antigen retrieval was performed using citrate buffer, pH 6.0, microwave, for 20min. Streptavidin-HRP Kit (Rabbit IgG) was used as the secondary antibody.
blocking condition: 5% BSA at Room Temperature for 1 hour
Chromagen system: DAB
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Immunofluorescence analysis of HeLa cells labelling Beclin 1 with ab62557 at 1/100 dilution. Cells were fixed with 4% paraformaldehyde at room temperature for 15 minutes and was permeabilized with 0.1% Triton X-100 at room temperature for 4 minutes. Rabbit IgG antibody (Alexa Fluor 488) at 1/2000 was used as the secondary antibody (green). Nuclear DNA was labelled with DAPI (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue labelling Beclin 1 with ab6257 at 1 µg/mL. Heat mediated antigen retrieval was performed using citrate buffer, pH 6.0, microwave, for 20min. Streptavidin-HRP Kit (Rabbit IgG) was used as the secondary antibody.
blocking condition: 5% BSA at Room Temperature for 1 hour
Chromagen system: DAB
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All lanes : Anti-Beclin 1 antibody (ab62557) at 1 µg/ml
Lane 1 : Mouse heart tissue
Lane 2 : Mouse brain tissue
Lane 3 : Mouse colon tissue
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Rabbit IgG antibody (HRP) at 1/10000 dilution
Predicted band size: 52 kDaGel: 10%
Running condition: 130V, 1.5hrs
Transfer condition: Wet, 250mA, 2 hrs (NC membrane)
Blocking condition: 5% non-fat milk in TBS at 4 overnight
Primary antibody incubation: RT, 1hr
Secondary antibody incubation: RT, 1hr
Washing condition: 5 ml TBST, 4 x 5min
Exposure system: GE ECL 10 sec
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All lanes : Anti-Beclin 1 antibody (ab62557) at 1 µg
Lane 1 : Rat liver tissue
Lane 2 : Rat heart tissue
Lane 3 : Rat lung tissue
Lane 4 : Rat brain tissue
Lane 5 : Rat ovary tissue
Lane 6 : Rat colon tissue
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Rabbit IgG antibody (HRP) at 1/10000 dilution
Predicted band size: 52 kDaGel: 10%
Running condition: 130V, 1.5hrs
Transfer condition: Wet, 250mA, 2 hrs (NC membrane)
Blocking condition: 5% non-fat milk in TBS at 4 overnight
Primary antibody incubation: RT, 1hr
Secondary antibody incubation: RT, 1hr
Washing condition: 5 ml TBST, 4 x 5min
Exposure system: GE ECL 10 sec
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat spleen tissue labelling Beclin 1 with ab6257 at 1 µg/mL. Heat mediated antigen retrieval was performed using citrate buffer, pH 6.0, microwave, for 20min. Streptavidin-HRP Kit (Rabbit IgG) was used as the secondary antibody.
blocking condition: 5% BSA at Room Temperature for 1 hour
Chromagen system: DAB
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ab62557 at 20 µg/mL staining Beclin 1 (green) in mouse brain tissue by Immunofluorescence. DAPI staining in blue.
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Paraffin-embedded human brain tissue stained for Beclin 1 using ab62557 at 2.5 µg/ml in immunohistochemical analysis.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (266)
ab62557 has been referenced in 266 publications.
- Zhang Z et al. Portulaca oleracea L. Extract Ameliorates Intestinal Inflammation by Regulating Endoplasmic Reticulum Stress and Autophagy. Mol Nutr Food Res 66:e2100791 (2022). PubMed: 34968000
- Jiang Y et al. Vitexin Attenuates Non-alcoholic Fatty Liver Disease Lipid Accumulation in High Fat-Diet Fed Mice by Activating Autophagy and Reducing Endoplasmic Reticulum Stress in Liver. Biol Pharm Bull 45:260-267 (2022). PubMed: 35034930
- Xiang J et al. Salvianolic acid B alleviates diabetic endothelial and mitochondrial dysfunction by down-regulating apoptosis and mitophagy of endothelial cells. Bioengineered 13:3486-3502 (2022). PubMed: 35068334
- Zheng X et al. TAK1 accelerates transplant arteriosclerosis in rat aortic allografts by inducing autophagy in vascular smooth muscle cells. Atherosclerosis 343:10-19 (2022). PubMed: 35078016
- Chen X et al. Anti-inflammatory effect of baicalin in rats with adjuvant arthritis and its autophagy- related mechanism. Technol Health Care 30:191-200 (2022). PubMed: 35124596