Anti-Beclin 1 (phospho S234) antibody (ab183335)
Key features and details
- Rabbit polyclonal to Beclin 1 (phospho S234)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Beclin 1 (phospho S234) antibody
See all Beclin 1 primary antibodies -
Description
Rabbit polyclonal to Beclin 1 (phospho S234) -
Host species
Rabbit -
Specificity
Immunolabeling of the Beclin 1 band is decreased in the Beclin 1 alanine substitution mutant. Additional bands may be observed in non-immunoprecipitated lysates. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Cow, Dog, Non human primates -
Immunogen
Synthetic peptide corresponding to Beclin 1 (phospho S234). The phosphopeptide corresponds to amino acid residues surrounding phospho-serine 234.
Database link: Q14457 -
Positive control
- HeLa and 293T expressing recombinant Beclin 1 cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.50
Constituents: 0.24% HEPES, 0.88% Sodium chloride, 50% Glycerol, 0.01% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab183335 was prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity column. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183335 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/250. Detects a band of approximately 60 kDa (predicted molecular weight: 52 kDa).
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Notes |
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WB
1/250. Detects a band of approximately 60 kDa (predicted molecular weight: 52 kDa). |
Target
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Function
Plays a central role in autophagy (By similarity). May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the beclin family. -
Cellular localization
Golgi apparatus > trans-Golgi network membrane. Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells. - Information by UniProt
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Database links
- Entrez Gene: 527278 Cow
- Entrez Gene: 8678 Human
- Entrez Gene: 56208 Mouse
- Entrez Gene: 114558 Rat
- Entrez Gene: 100913170 Sheep
- Omim: 604378 Human
- SwissProt: Q4A1L4 Cow
- SwissProt: Q14457 Human
see all -
Alternative names
- APG6 antibody
- ATG 6 antibody
- ATG6 antibody
see all
Images
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All lanes : Anti-Beclin 1 (phospho S234) antibody (ab183335) at 1/250 dilution
Lane 1 : Lysate from 293T cells expressing wild type flag-Beclin 1
Lane 2 : Lysate from 293T cells expressing mutant flag-Beclin 1 (S234A, S295A).
Predicted band size: 52 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab183335 has been referenced in 7 publications.
- Xiong L et al. The protective effects of melatonin in high glucose environment by alleviating autophagy and apoptosis on primary cortical neurons. Mol Cell Biochem N/A:N/A (2022). PubMed: 36348200
- Ma M et al. miR-17-5p attenuates kidney ischemia-reperfusion injury by inhibiting the PTEN and BIM pathways. Ann Transl Med 9:1545 (2021). PubMed: 34790751
- Tan P et al. Rapamycin-induced miR-30a downregulation inhibits senescence of VSMCs by targeting Beclin1. Int J Mol Med 43:1311-1320 (2019). PubMed: 30747228
- Kumar A & Shaha C SESN2 facilitates mitophagy by helping Parkin translocation through ULK1 mediated Beclin1 phosphorylation. Sci Rep 8:615 (2018). PubMed: 29330382
- Huang G et al. Notoginsenoside R1 attenuates glucose-induced podocyte injury via the inhibition of apoptosis and the activation of autophagy through the PI3K/Akt/mTOR signaling pathway. Int J Mol Med 39:559-568 (2017). Human . PubMed: 28112381
- Liu W et al. Electroacupuncture protects against ischemic stroke by reducing autophagosome formation and inhibiting autophagy through the mTORC1-ULK1 complex-Beclin1 pathway. Int J Mol Med 37:309-18 (2016). WB . PubMed: 26647915
- Li C et al. Molecular switch role of Akt in Polygonatum odoratum lectin-induced apoptosis and autophagy in human non-small cell lung cancer A549 cells. PLoS One 9:e101526 (2014). WB ; Human . PubMed: 24992302