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Beta Glucuronidase (GUS) protein (Active) (ab58415)

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Overview

  • Product nameBeta Glucuronidase (GUS) protein (Active)
  • Protein descriptionRecombinant full length beta Glucuronidase protein (Escherichia coli )
  • Expression hostE. coli

    • Properties

  • Purification notesComposition is 50% protein by biuret.
  • Biological activityActivity: >10,000,000 units/gm protein. This enzyme does not hydrolyze alpha-glucuronides or beta-glucosides. This preparation is essentially free of sulfatase activity.
  • FormLyophilised:Reconstitute with 75mM phosphate buffer, pH 6.8, to give a 5mg/ml solution. This solution will be clear to slightly hazy but is still active regardless.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferPreservative: None
    Constituents: Polyethylene glycol (as stabilizer), 10mM Potassium phosphate, 1mM EDTA, 1mM DTT
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab58415 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS: Use at an assay dependent dilution. beta-Glucuronidase Activity: One unit will liberate 1.0 mg of phenolphthalein from phenolphthalein glucuronide per hour at 37 °C at pH 6.8 (30 minute assay). Optimal pH: 6-7.

    Protein info

    • Alternative names
        Beta D glucuronidaseBeta G1Beta glucuronidase
        FLJ39445Glucuronidase betagurAGUSBMPS 7MPS7UidA
      see all
  • RelevanceReporter genes are widely used for studying the expression of foreign genes in transformed plants tissues. Using appropriate promoter-reporter gene constructs, this technique allows an independent verification of the transformed status of tissues growing on media containing selective antibiotics or herbicides. In addition, it serves as a principal means to follow gene transfer and monitor genetic transformation of plant species. Encoded by the E. coli GUS gene (also referred to as uidA), GUS protein is a hydrolase that catalyses the cleavage of a variety of beta-glucuronide derivatives available for colorimetric, fluorimetric and histochemical assays. Several features make the gus gene superior as a reporter gene for plant studies and in the production of genetically engineered crops.
  • Cellular localizationCytoplasmic

    • References for Beta Glucuronidase (GUS) protein (Active) (ab58415)

    ab58415 has not yet been referenced specifically in any publications.

    Publishing research using ab58415 ? Please let us know so that we can cite the reference in this datasheet

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    What is the mass of protein provided in a vial of lot GR55580-1?

    Thank you for your enquiry regarding ab58415. I'm sorry for the delay in responding to your question. This lot contained 17,400,000 units per g of protein, protein was 45% of the total mass of the solid. So for this lot of ab58415, one vial of 25,000 unit...

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    I need to have the protein or DNA sequence for this item in order to order it. It would be great if you can provide me this info.

    Our production group prepared a synthetic DNA made from the sequence of the uidA gene from the E. coli K12 strain. The UniProtKB/Swiss-Prot entry for the uidA gene sequence is P05804. Here is the FASTA protein sequence: >sp|P05804|BGLR_ECOLI Beta...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"