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Overview

  • Product nameβNGF Rat ELISA KitSee all Beta nerve growth factor kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Platelets
  • Assay typeSandwich
  • Sensitivity
    < 40 pg/ml
  • Range
    0.049 ng/ml - 100 ng/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 132.1 123% - 142%
    Serum 111.7 102% - 121%
    Plasma 97.02 70% - 126%

  • Species reactivity
    Reacts with: Rat
  • Product overview

    ab100757 NGF Rat (beta-nerve growth factor) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat NGF in serum, plasma and cell culture supernatants. This assay employs an antibody specific for NGF coated on a 96-well plate. Standards and samples are pipetted into the wells and NGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat NGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of NGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsELISA more details

Properties

  • RelevanceNerve growth factor (NGF) is one of a family of neurotrophins that induce the survival and proliferation of neurons. In cell culture, NGF induces the formation of neurite projections and, in vivo, may stimulate the innervation of tissues. NGF plays a role in the repair, regeneration, and protection of neurons, and as such could serve as a therapeutic agent in neurodegenerative conditions such as Alzheimer's disease. NGF enhances survival, growth, neurotransmitter biosynthesis of sympathetic and sensory neurons; neurotrophic factor; cutaneous innervation; growth, differentiation and survival of B lymphocytes. It also has a possible role in allergy and tissue repair. NGF is found in the hypothalamus, pituitary, thyroid gland, testes, epididymis, vascular smooth muscle cells, fibroblasts, mast cells and eosinophils. NGF is upregulated by glutamate, vitamin D3, IL6, FGF basic, astrocyte specific IL1, TNF alpha, PDGF and TGF beta. It is downregulated by GABAergic neuronal activity, glucocorticoids and Schwann cell-specific TGF beta.
    • Alternative names
        Beta nerve growth factorBeta NGFHSAN5
        MGC161426MGC161428Nerve growth factor (beta polypeptide)Nerve growth factor beta polypeptideNerve growth factor beta subunitNGFBNID67
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab100757 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      βNGF Rat ELISA Kit images

      • ELISA - Beta NGF Rat ELISA Kit - 1 x 96 Well Plate (ab100757)
        ELISA - Beta NGF Rat ELISA Kit - 1 x 96 Well Plate (ab100757)

      Protocols

      References for βNGF Rat ELISA Kit (ab100757)

      This product has been referenced in:
      • Liu J  et al. Contribution of nerve growth factor to upregulation of P2X3 expression in DRG neurons of rats with femoral artery occlusion. Am J Physiol Heart Circ Physiol 301:H1070-9 (2011). Read more (PubMed: 21642505) »

      See 1 Publication for this product

      Publishing research using ab100757 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab100757 Submit a Question for ab100757

      Displaying 1 - 7 of 7 results for Abreviews and Q&A

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      Votes: Highest First

      May I check what's the detectable range for ab100757 Beta NGF Rat ELISA kit please? On the website and SOP, you indicated it's 0.049ng/mL to 100ng/mL, but the standards in this kit ranges from 0.02058ng/mL to 15ng/mL. Also, is it feasible to use my own ...

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      Thank you for contacting us. The minimum detectable dose of beta-NGF is typically less than 15 pg/ml with this kit. We present this range with the more conservative low point of 49pg/ml to ensure accurate data. Regarding standards, the included standar...

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      I got a beta NGF rat ELISA kit (ab100757)

      Can you please advise me the initial concentration of standards (is it 50ng/mL after adding 400uL of Diluent B into Item C)?

      Also, what’s the region of detectable concentration?

      Thank you for your enquiry.

      Regarding question 1: Yes you are correct. The initial concentration of the resuspended standard will be 50ng/ml.

      Regading the region of detectable concentration: The minimum detectable dose of NGF is typica...

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      I ordered this kit, but I am missing the 2 vials of item F (Detection antibody NGF)and the 1 vial of item G (HRP-Streptavidin Concentrate).

      Thank you for alerting us to this problem.


      We will be sending you 2 vials of item F and 1 vial of item G.

      I am sorry about the mix-up, but I hope we can get this squared away for you. Please let me know if you have any other que...

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      I am using rat serum sampleswith this kit and in some wells there is precipitation after addition of the stop solution. Id does not happen in all of the wells, only a few and the wells that it does occur in, have a lower reading compared to others. It als...

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      Thank you for contacting Abcam. I have had a reposnse from the lab about the issues you were having with ab100757. Please see their response below: Were the wells with precipitate also “dark blue” before adding the Stop Solution? If so, this l...

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      I am using rat serum sampleswith this kit and in some wells there is precipitation after addition of the stop solution. Id does not happen in all of the wells, only a few and the wells that it does occur in, have a lower reading compared to others. It als...

      Read More

      Thank you for contacting Abcam. I just wanted to let you know that I am still waiting to hear back from the lab about the problems you have been having with ab100757 and I will contact you again, as soon as I hear back from them. I do appreciate your pati...

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      Can this kit be used with urine? Are there any special instructions?

      Thank you for your inquiry.  While ab100757 has not been validated with urine (only serum, plasma, and cell culture supernatants), our ELISA kits are very accommodating to many different types of samples so urine along with other body fluids will likely b...

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      Can tissue homogenate be used as a sample with this kit?

      While this kit has only been validated with serum, plasma, and cell culture supernatants, it is very accommodating and should work with most sample types including cell and tissue lysates. Attached are some general tips to help the customer prepare ...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"