Anti-Beta TRCP antibody (ab137674)
Key features and details
- Rabbit polyclonal to Beta TRCP
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Beta TRCP antibody
See all Beta TRCP primary antibodies -
Description
Rabbit polyclonal to Beta TRCP -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, Xenopus tropicalis -
Immunogen
Recombinant fragment corresponding to Human Beta TRCP aa 50-400.
Database link: Q9Y297 -
Positive control
- Recombinant Human Beta TRCP protein (ab132456) can be used as a positive control in WB. 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137674 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 68 kDa.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 68 kDa. |
Target
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Function
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds to phosphorylated target proteins. SCF(BTRC) mediates the ubiquitination of CTNNB1 and participates in Wnt signaling. SCF(BTRC) mediates the ubiquitination of NFKBIA, NFKBIB and NFKBIE; the degradation frees the associated NFKB1 to translocate into the nucleus and to activate transcription. Ubiquitination of NFKBIA occurs at 'Lys-21' and 'Lys-22'. SCF(BTRC) mediates the ubiquitination of phosphorylated NFKB1/nuclear factor NF-kappa-B p105 subunit, ATF4, SMAD3, SMAD4, CDC25A, DLG1, FBXO5 and probably NFKB2. SCF(BTRC) mediates the ubiquitination of phosphorylated SNAI1. May be involved in ubiquitination and subsequent proteasomal degradation through a DBB1-CUL4 E3 ubiquitin-protein ligase. Required for activation of NFKB-mediated transcription by IL1B, MAP3K14, MAP3K1, IKBKB and TNF. Required for proteolytic processing of GLI3. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 F-box domain.
Contains 7 WD repeats. -
Domain
The N-terminal D domain mediates homodimerization. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 534072 Cow
- Entrez Gene: 8945 Human
- Entrez Gene: 12234 Mouse
- Entrez Gene: 361765 Rat
- Omim: 603482 Human
- SwissProt: Q9Y297 Human
- SwissProt: Q3ULA2 Mouse
- Unigene: 643802 Human
see all -
Alternative names
- Beta transducin repeat containing E3 ubiquitin protein ligase antibody
- beta TrCP1 antibody
- beta-transducin repeat containing antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab137674 has been referenced in 3 publications.
- Liu Y et al. Protective effect of endothelial progenitor cell-derived exosomal microRNA-382-3p on sepsis-induced organ damage and immune suppression in mice. Am J Transl Res 14:6856-6873 (2022). PubMed: 36398226
- Collart C et al. Chk1 Inhibition of the Replication Factor Drf1 Guarantees Cell-Cycle Elongation at the Xenopus laevis Mid-blastula Transition. Dev Cell 42:82-96.e3 (2017). PubMed: 28697335
- McCormack RM et al. Enteric pathogens deploy cell cycle inhibiting factors to block the bactericidal activity of Perforin-2. Elife 4:N/A (2015). WB . PubMed: 26418746