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Betacellulin (BTC) Human ELISA Kit (ab99987)

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Overview

  • Product nameBetacellulin (BTC) Human ELISA Kit
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 30 pg/ml
  • Range
    24.69 pg/ml - 18000 pg/ml
  • Recovery

    80 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 74.34 65% - 85%
    Serum 96.23 87% - 105%
    Plasma 78.86 67% - 88%

  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Human Betacellulin (BTC) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Betacellulin in serum (Human Betacellulin concentration is pretty low in normal serum/plasma, it may not be detected in this assay), plasma, cell culture supernatants.

    This assay employs an antibody specific for Human Betacellulin coated on a 96-well plate. Standards and samples are pipetted into the wells and Betacellulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Betacellulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Betacellulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsELISA more details

Properties

  • Storage instructionsStore at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    20X Wash Buffer Concentrate Item B 1 x 25ml
    240X HRP-Streptavidin Concentrate Item G 1 x 200µl
    Assay Diluent B 5x concentrated buffer. For detection antibody and HRP- Streptavidin diluent. 1 x 15ml
    Assay Diluent D 5x concentrated buffer. For Standard/Sample (serum/plasma samples/ cell culture medium/urine) diluent. 1 x 15ml
    BTC Microplate (12 strips x 8 wells) coated with anti-human BTC. 1 unit
    Detection Antibody BTC: biotinylated anti-human BTC 2 vials
    Human BTC Standard (recombinant) 2 vials
    Stop Solution Item I 1 x 8ml
    TMB One-Step Substrate Reagent Item H 1 x 12ml
  • Research Areas
  • RelevanceThe protein encoded by this gene is a member of the mammalian members of the EGF family of growth factors. It is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This encoded protein is a ligand for the EGF receptor. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
    • Alternative names
        Betacellulin precursorBTCProbetacellulin
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab99987 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      Betacellulin (BTC) Human ELISA Kit images

      • ELISA Typical Data
        ELISA Typical Data

        Representative Standard Curve using ab99987.

      • ELISA - Betacellulin Human ELISA Kit (ab99987)
        ELISA - Betacellulin Human ELISA Kit (ab99987)

      Protocols

      References for Betacellulin (BTC) Human ELISA Kit (ab99987)

      ab99987 has not yet been referenced specifically in any publications.

      Publishing research using ab99987 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab99987 Submit a Question for ab99987

      Displaying 1 - 4 of 4 results for Abreviews and Q&A

      10
      Votes: Highest First

      I want to order ELISA kits for a few ligands, eg: . HB EGF Human ELISA Kit (ab100531), TGF alpha Human ELISA Kit (ab100646), Betacellulin Human ELISA Kit. All these are 96 reactions each. I want only a few wells for each ligand. Can I buy one kit and meas...

      Read More

      Thank you for your interest in our ELISA kits. Each kit comes with a microplate that is already coated with an antibody specific for the the protein of interest, so you would not be able to use one plate to detect all three proteins (EGF, TGF, betacelluli...

      Read More

      What is the concentration in Human plasma/serum?

      We have not tested human plasma/serum concentration but the following publication does mention that 40ng/ml （40,000pg/ml） concentration is found in human plasma.



      Betacellulin Induces Increased Retinal Vascular Permeability in Mice. Read More

      Dear,
      I have a technical problem with the Abcam ELISA kit for Human HB-EGF
      (ab100531) and Human Betacellulin (ab99987). For these 2 ELISAs, I got
      very low signals for the standard curves.
      Since I am also working with other Abcam ELISA ...

      Read More

      Thank you for contacting us.

      I am very sorry to hear these kits failed to give the expected results. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

      In order to bet...

      Read More

      A customer has the following enquiry regarding this kit;

      In regards to the “Betacellulin Human ELISA Kit”(ab99987) below, do you have any info available on the concentration range of this protein in normal human plasma and/or serum? O...

      Read More

      Thank you for contacting us.

      Unfortunately wearenot able to find a publication stating what the normal levels of betacellulin in human serum/plasma was either. Based on our QC tests on the kit with normal human serum and plasma samples, the measu...

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"