Betacellulin (BTC) Human ELISA Kit (ab99987)
- Product nameBetacellulin (BTC) Human ELISA Kit
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 30 pg/ml
- Range24.69 pg/ml - 18000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 74.34 65% - 85% Serum 96.23 87% - 105% Plasma 78.86 67% - 88%
- Species reactivityReacts with: Human
- Product overview
Abcam’s Human Betacellulin (BTC) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Betacellulin in serum (Human Betacellulin concentration is pretty low in normal serum/plasma, it may not be detected in this assay), plasma, cell culture supernatants.
This assay employs an antibody specific for Human Betacellulin coated on a 96-well plate. Standards and samples are pipetted into the wells and Betacellulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Betacellulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Betacellulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Wash Buffer Concentrate Item B 1 x 25ml 240X HRP-Streptavidin Concentrate Item G 1 x 200µl Assay Diluent B 5x concentrated buffer. For detection antibody and HRP- Streptavidin diluent. 1 x 15ml Assay Diluent D 5x concentrated buffer. For Standard/Sample (serum/plasma samples/ cell culture medium/urine) diluent. 1 x 15ml BTC Microplate (12 strips x 8 wells) coated with anti-human BTC. 1 unit Detection Antibody BTC: biotinylated anti-human BTC 2 vials Human BTC Standard (recombinant) 2 vials Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml
- Research Areas
- Betacellulin precursorBTCProbetacellulin
- SwissProt: P35070 Human
Our Abpromise guarantee covers the use of ab99987 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Betacellulin (BTC) Human ELISA Kit (ab99987)
ab99987 has not yet been referenced specifically in any publications.