Betacellulin antibody [34362.11] (ab10417)

Product Name 

Betacellulin antibody [34362.11]

Product type 

Primary antibodies

Description 

Mouse monoclonal [34362.11] to Betacellulin

Immunogen 

Recombinant full length protein (Human).

Reacts with 
(species key)

Hu

Specificity 

Does not cross-react with recombinant human EGF, HB-EGF, TGF-alpha, HRG-alpha and AR.

Tested applications 
(see key)

ELISA, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: Use at a concentration of 1 - 2 µg/ml.
WB: Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 32 kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Precursor form: Cell membrane; single-pass type I membrane protein. Mature form: Secreted protein; extracellular space.

Research areas 

Signal Transduction >> Growth Factors/Hormones >> EGF

Relevance 

The protein encoded by this gene is a member of the mammalian members of the EGF family of growth factors. It is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This encoded protein is a ligand for the EGF receptor. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.


Betacellulin (BTC) is a heparin-binding member of the EGF family of growth factors that includes epidermal growth factor (EGF), transforming growth factor-alpha (TGF-alpha), amphiregulin (AR), heparin-binding EGF-like growth factor (HB-EGF), and the various heregulins (HRG). These growth factors bind to the EGF receptor and share at least 28 % sequence identity and 100% conservation of the 6 cysteine residue positions in the mature peptide sequence. Recombinant human betacellulin cDNA encodes an 80 amino acid residue polypeptide 7 with a predicted molecular mass of approximately 9.5 kDa. Betacellulin (BTC) was originally isolated as a 32 kDa glycoprotein from the conditioned media of mouse pancreatic beta tumor cells. It is capable of stimulating the proliferation of 3T3 cells, retinal pigment epithelial cells, and smooth muscle cells. The cDNA that encodes human BTC was cloned from a human breast adenocarcinoma cell line (MCF-7). Betacellulin is synthesized as a transmembrane precursor protein and then cleaved to release three types of soluble BTC forms (full length plus two amino-terminal truncation sites), all of which are equally bioactive. Human and mouse precursor forms of BTC exhibit 79 % amino acid sequence identity. Betacellulin is a unique EGF family member in that it binds with high affinity to EGF receptor, ErbB-1, and ErbB-4. Overexpression of betacellulin with ErbB-3 and ErbB-4 are linked to human endometrial adenocarcinoma. 2 Betacellulin has a role in the growth and differentiation of islet cells in the human pancreas.5 Betacellulin is produced in a wide variety of tissues, cultured cells, and various body fluids including milk. It is highly expressed in pancreas as well as liver, kidney, and small intestine. Betacellulin can also be found in lower abundance in testis, ovary, lung, and colon. BTC mRNA has not been found in brain, placenta, spleen, thymus, or peripheral blood leukocytes.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

34362.11

Isotype 

IgG1

Purity 

Protein A purified

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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