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Product name
Anti-Bid Cleavage Site antibody
See all Bid Cleavage Site products (2) ...
Description
Rabbit polyclonal to Bid Cleavage Site
Tested applications
Cross reactivity
Reacts with
Mouse
Does not react with
Human
Immunogen
Synthetic peptide (Mouse)corresponding to N-terminus of cleavage site (59/60).
Positive control
TNF alpha treated L929 cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from rabbit serum by epitope-specific affinity chromatography.
Primary antibody notes
BH3 interacting domain death agonist (BID) is a pro-apoptotic member of the Bcl 2 family. BID interacts with both Bcl 2 and Bax through its BH3 domain. It usually exists in an inactive form in the cytosolic fraction of living cells and becomes cleaved and activated by caspase 8 in response to TNF alpha or Fas ligand. Once BID is cleaved, the C-terminal 15 kDa fragment of BID (p15) translocates onto mitochondria and is sufficient to trigger cytochrome c release, resulting in cell apoptosis. BID serves as a direct molecular link between caspase 8 activation and mitochondrial death machinery.
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Pathways and Processes >> Metabolism processes >> Apoptosis
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Cell Biology >> Apoptosis >> Mitochondrial
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
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Western blot - Bid Cleavage Site antibody (ab10640)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab10640 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 15 kDa.
Target
Relevance
Bid, a BH3 domain containing proapoptotic Bcl2 family member, is localized in the cytosolic fraction of cells as an inactive precursor. Its active form is generated upon proteolytic cleavage by caspase 8 in the Fas signaling pathway. Cleaved Bid translocates to mitochondria and releases its potent proapoptotic activity, which in turn induces cytochrome c release and mitochondrial damage. The cytochrome c releasing activity of Bid was antagonized by Bcl2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In animal model studies, Bid deficient mice are found resistant to the lethal effects of death factor signals relayed through Fas.
Alternative names
- Apoptic death agonist antibody
- BH 3 interacting domain death agonist antibody
- BH3 Interacting Domain Death Agonist antibody
see all
Database links
- Entrez Gene: 12122 Mouse
- SwissProt: P70444 Mouse
- Unigene: 34384 Mouse
Anti-Bid Cleavage Site antibody images:
Western blot - Bid Cleavage Site antibody (ab10640)
Predicted band size : 15 kDa
Mouse L929 cells were treated with 5 µg/mL actinomycin plus 2.5 ng/mL, 5 ng/mL and 10 ng/mL mouse TNF alpha for 5 hours (Lane 3, 4 and 5). Untreated L929 cells (Lane 1) and cells treated with actinomycin alone (Lane 2) were used as controls. Proteins were resolved from the cells by SDS-PAGE. The proteins were transferred to PVDF membranes. The membranes were incubated with the anti-mouse full length BID antibody (left panel) and ab10640 (right panel) at 1 µg/mL. The signal was detected using a goat F(ab’)2 anti-rabbit IgG alkaline phosphatase antibody at a 1/5000 dilution and the membrane was incubated with CDP-substrate followed by chemiluminescence. The data show that ab10640 only recognizes the cleaved mouse BID and not full length (Right panel). The full length BID was revealed using an anti-mouse full length BID antibody (Left panel).
Protocols
References for Anti-Bid Cleavage Site antibody (ab10640)
ab10640 has not yet been referenced specifically in any publications.
Publishing research using ab10640? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"