Anti-Bid antibody (ab62469)
Key features and details
- Rabbit polyclonal to Bid
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Bid antibody
See all Bid primary antibodies -
Description
Rabbit polyclonal to Bid -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
A 14 amino acid synthetic peptide from near the carboxy-terminus of human Bid
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Positive control
- Mouse lung cell lysates and mouse lung tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Ion Exchange Chromatography -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab62469 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 20 µg/ml.
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WB | (2) |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 22 kDa).
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IHC-P |
Use a concentration of 2 µg/ml.
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Notes |
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ICC/IF
Use a concentration of 20 µg/ml. |
WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 22 kDa). |
IHC-P
Use a concentration of 2 µg/ml. |
Target
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Function
The major proteolytic product p15 BID allows the release of cytochrome c (By similarity). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2. -
Tissue specificity
Isoform 2 and isoform 3 are expressed in spleen, bone marrow, cerebral and cerebellar cortex. Isoform 2 is expressed in spleen, pancreas and placenta (at protein level). Isoform 3 is expressed in lung, pancreas and spleen (at protein level). Isoform 4 is expressed in lung and pancreas (at protein level). -
Domain
Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family. -
Post-translational
modificationsTNF-alpha induces a caspase-mediated cleavage of p22 BID into a major p15 and minor p13 and p11 products.
Phosphorylated upon DNA damage, probably by ATM or ATR.
p15 BID is ubiquitinated by ITCH; ubiquitination results in proteasome-dependent degradation. -
Cellular localization
Cytoplasm; Cytoplasm. Mitochondrion membrane. When uncleaved, it is predominantly cytoplasmic; Mitochondrion membrane. A significant proportion of isoform 2 localizes to mitochondria, it may be cleaved constitutively; Mitochondrion membrane. Associated with the mitochondrial membrane and Mitochondrion membrane. Translocates to mitochondria as an integral membrane protein. - Information by UniProt
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Database links
- Entrez Gene: 12122 Mouse
- SwissProt: P70444 Mouse
- Unigene: 235081 Mouse
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Alternative names
- Apoptic death agonist antibody
- Apoptotic death agonist BID antibody
- BH3 interacting domain death agonist antibody
see all
Images
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Lane 1 : Anti-Bid antibody (ab62469) at 0.5 µg/ml
Lane 2 : Anti-Bid antibody (ab62469) at 1 µg/ml
Lane 3 : Anti-Bid antibody (ab62469) at 2 µg/ml
All lanes : Mouse lung cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Anti rabbit IgG secondary antibody
Predicted band size: 22 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted? -
Immunohistochemical staining of mouse lung tissue using ab62469 antibody at 2 µg/ml.
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Immunofluorescence of Bid in Mouse Lung cells using ab62469 at 10 ug/ml.
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All lanes : Anti-Bid antibody (ab62469) at 1/1000 dilution
Lane 1 : HCT116 colon cancer cell line treated with DMSO (control)
Lane 2 : HCT116 colon cancer cell line treated with 2 µM SAHA for 24 hrs
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 22 kDa
Exposure time: 10 seconds
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab62469 has been referenced in 6 publications.
- Pang D et al. Soy isoflavones improve the oxidative stress induced hypothalamic inflammation and apoptosis in high fat diet-induced obese male mice through PGC1-alpha pathway. Aging (Albany NY) 12:8710-8727 (2020). PubMed: 32434959
- Wang C et al. Isoforsythiaside Attenuates Alzheimer's Disease via Regulating Mitochondrial Function Through the PI3K/AKT Pathway. Int J Mol Sci 21:N/A (2020). PubMed: 32784451
- Kinsella S et al. Increased A20-E3 ubiquitin ligase interactions in bid-deficient glia attenuate TLR3- and TLR4-induced inflammation. J Neuroinflammation 15:130 (2018). PubMed: 29720226
- Barrott JJ et al. The Influential Role of BCL2 Family Members in Synovial Sarcomagenesis. Mol Cancer Res 15:1733-1740 (2017). PubMed: 28851813
- Sula Karreci E et al. Human regulatory T cells undergo self-inflicted damage via granzyme pathways upon activation. JCI Insight 2:N/A (2017). WB . PubMed: 29093262
- Kinsella S et al. Bid Promotes K63-Linked Polyubiquitination of Tumor Necrosis Factor Receptor Associated Factor 6 (TRAF6) and Sensitizes to Mutant SOD1-Induced Proinflammatory Signaling in Microglia. eNeuro 3:N/A (0). ICC/IF . PubMed: 27257617