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Anti-Bid antibody [Y8] (ab32060)

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1 question for ab32060

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Question 1

Thursday 03-November-2011

The preferred option would be to p15. But we need to be tested in immunocytochemistry.    

ANSWER:

  

Thank you for your reply. In terms of rabbit antibodies to Bid that have been tested in ICC, we have only ab32060 http://www.abcam.com/Bid-antibody-Y8-ab32060.html It has not been tested in mouse yet, though. The immunogen is a synthetic peptide corresponding to residues before the BH3 domain of human Bid (it is amino acids 50-62). So this will not recognize the p15 fragment resulting from cleavage at 61/62 or p11 or p13. See http://www.uniprot.org/uniprot/P55957 We also have a mouse monoclonal that was tested in ICC/IF (ab114051), but not in mouse. It was raised against a purified recombinant fragment of Human Bid expressed in E. coli, but I have no further information right now about the sequence covered by the immunogen. It seems that we might not have the exact product you are looking for. I might suggest checking also www.biocompare.com. I just wanted to point out that we do have a testing discount program for UNTESTED species and/or applications. Here is a brief description of how it works: The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. 

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