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Product Name
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Bid antibody
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See all Bid antibodies (14)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Bid
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Immunogen
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Synthetic peptide, surrounding amino acid 37 of Human Bid.
(Peptide available as ab38270.)
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Reacts with
(species key)
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Hu
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Specificity
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This antibody detects both full length (22 kDa) and cleaved fragments (15 kDa) of Bid.
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Tested applications
(see key)
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IHC-Fr, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-Fr: Use at a concentration of 20 - 40 µg/ml.
IP: Use at a concentration of 5 - 10 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 22 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Isoform 1 and isoform 3: Cytoplasm.
Isoform 2: Mitochondrial membrane.
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Research areas
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Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
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Relevance
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Bid, a BH3 domain containing proapoptotic Bcl2 family member, is localized in the cytosolic fraction of cells as an inactive precursor. Its active form is generated upon proteolytic cleavage by caspase 8 in the Fas signaling pathway. Cleaved Bid translocates to mitochondria and releases its potent proapoptotic activity, which in turn induces cytochrome c release and mitochondrial damage. The cytochrome c releasing activity of Bid was antagonized by Bcl2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In animal model studies, Bid deficient mice are found resistant to the lethal effects of death factor signals relayed through Fas.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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PBS with 1 mg/ml BSA, 1.5 mM sodium azide and 50% glycerol Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Bid antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Bid antibody images, references, products related to ab2388 and other tools.
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Bid antibody images:
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 - Bid antibody (ab2388)
Western blot analysis of Bid in Jurkat cells treated with anti-Fas for 6 hours.
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Search PubMed (MEDLINE) for references to Bid
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Bid antibody - more information
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Customer reviews (feedback) regarding Bid antibody |
Customer FAQs regarding Bid antibody |
Protocols for Bid antibody |
Price and availability of products related to Bid antibody |
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Bid antibody - related products:
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Bid antibody - other tools:
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Contact Abcam with a Technical Enquiry about Bid antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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