Bid antibody (ab2561)

Product Name 

Bid antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Bid

Immunogen 

Synthetic peptide surrounding amino acid 45 of Mouse Bid.

Reacts with 
(species key)

Ms

Specificity 

This antibody detects both full length (22 kDa) and cleaved fragment (15 kDa) of mouse Bid.

Tested applications 
(see key)

IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
IP: Use at a concentration of 20 µg/ml.
WB: Use at a concentration of 1.0 µg/ml.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Isoform 1 and isoform 3: Cytoplasm.
Isoform 2: Mitochondrial membrane.

Research areas 

Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family

Relevance 

Bid, a BH3 domain containing proapoptotic Bcl2 family member, is localized in the cytosolic fraction of cells as an inactive precursor. Its active form is generated upon proteolytic cleavage by caspase 8 in the Fas signaling pathway. Cleaved Bid translocates to mitochondria and releases its potent proapoptotic activity, which in turn induces cytochrome c release and mitochondrial damage. The cytochrome c releasing activity of Bid was antagonized by Bcl2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In animal model studies, Bid deficient mice are found resistant to the lethal effects of death factor signals relayed through Fas.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

PBS pH7.2 with 50% glycerol, 1% BSA and 0.02% sodium azide

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

References for Bid antibody (ab2561)

  This product has been used in: (two most recent references)

McKernan DP & Cotter TG A Critical role for Bim in retinal ganglion cell death. J Neurochem 102:922-30 (2007). PubMed: 17442051

Emeterio EP et al. Modulation of apoptosis in the mouse brain after morphine treatments and morphine withdrawal. J Neurosci Res 83:1352-61 (2006). PubMed: 16496378
If you publish research using ab2561 please let us know so that we can cite the reference on this datasheet.

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