Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
Overview
Product name
Anti-Bid antibody
See all Bid products (16) ...
Description
Rabbit polyclonal to Bid
Tested applications
IHC-Pmore details
Cross reactivity
Reacts with
Human
Does not react with
Mouse
Immunogen
Synthetic peptide: SESQEDIIRN, corresponding to amino acids 76-85 of Human Bid
SESQEDIIRN
Positive control
Human colon carcinoma tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 50% Glycerol, 0.05M Sodium phosphate
Concentration
Concentration information loading...
Purification notes
ab58787 is purified.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
-
Immunohistochemistry (Paraffin-embedded sections) - Bid antibody (ab58787)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab58787 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
IHC-P
Application notes
IHC-P: Use at a concentration of 2.5 µg/ml. Heat pretreatment is required.
Is unsuitable for WB.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
The major proteolytic product p15 BID allows the release of cytochrome c (By similarity). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.
Tissue specificity
Isoform 2 and isoform 3 are expressed in spleen, bone marrow, cerebral and cerebellar cortex. Isoform 2 is expressed in spleen, pancreas and placenta (at protein level). Isoform 3 is expressed in lung, pancreas and spleen (at protein level). Isoform 4 is expressed in lung and pancreas (at protein level).
Domain
Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
Post-translational
modifications
TNF-alpha induces a caspase-mediated cleavage of p22 BID into a major p15 and minor p13 and p11 products.
Phosphorylated upon DNA damage, probably by ATM or ATR.
p15 BID is ubiquitinated by ITCH; ubiquitination results in proteasome-dependent degradation.
Cellular localization
Cytoplasm; Cytoplasm. Mitochondrion membrane. When uncleaved, it is predominantly cytoplasmic; Mitochondrion membrane. A significant proportion of isoform 2 localizes to mitochondria, it may be cleaved constitutively; Mitochondrion membrane. Associated with the mitochondrial membrane and Mitochondrion membrane. Translocates to mitochondria as an integral membrane protein.
Target information above from: UniProt accessionP55957
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Apoptic death agonist antibody
- Apoptotic death agonist antibody
- Apoptotic death agonist BID antibody
see all
Database links
- Entrez Gene: 637 Human
- Omim: 601997 Human
- SwissProt: P55957 Human
- Unigene: 591054 Human
Anti-Bid antibody images:
Immunohistochemistry (Paraffin-embedded sections) - Bid antibody (ab58787)
Detection of human Bid by staining paraffin sections.
Sample: Human colon carcinoma.
Primary antibody: ab58787 at 2.5 µg/ml.
Detection: DAB with methyl green counterstain.
References for Anti-Bid antibody (ab58787)
ab58787 has not yet been referenced specifically in any publications.
Publishing research using ab58787? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"