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Western blot - Bid antibody (ab63541)
(enlarge)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Bid antibody (ab63541)
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Product Name
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Bid antibody
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See all Bid antibodies (14)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Bid
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Immunogen
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Synthetic non-phosphopeptide derived from human Bid around the phosphorylation site of serine 78 (S-E-SP-Q-E).
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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ELISA, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/10000.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 22 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Jurkat cell. Brain tissue.
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Cellular localization
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Isoform 1 and isoform 3: Cytoplasm.
Isoform 2: Mitochondrial membrane.
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Research areas
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Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
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Relevance
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Bid, a BH3 domain containing proapoptotic Bcl2 family member, is localized in the cytosolic fraction of cells as an inactive precursor. Its active form is generated upon proteolytic cleavage by caspase 8 in the Fas signaling pathway. Cleaved Bid translocates to mitochondria and releases its potent proapoptotic activity, which in turn induces cytochrome c release and mitochondrial damage. The cytochrome c releasing activity of Bid was antagonized by Bcl2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In animal model studies, Bid deficient mice are found resistant to the lethal effects of death factor signals relayed through Fas.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca 2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Bid antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Bid antibody images, references, products related to ab63541 and other tools.
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Bid antibody images:
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 Western blot - Bid antibody (ab63541)
All lanes : Bid antibody (ab63541) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells (5ug) treated
with H2O2 (100uM, 30mins)
Lane 2 : Extracts from Jurkat cells (5ug) treated with etoposide (25uM, 60 mins)
Lane 3 : Extracts from Jurkat cells (5ug) treated
with H2O2 (100uM, 30mins) and etoposide (25uM, 60 mins) with immunizing non-phosphopeptide at 5 µg
Predicted band size : 22 kDa
Observed band size : 26 kDa (why is the actual band size different from the predicted?)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Bid antibody (ab63541)
Ab63541 (1:50) staining human Bid in human brain tissue by immunohistochemistry using formalin fixed, paraffin embedded tissue.
The left hand panel is without the presence of blocking peptide, whereas the right hand panel is in the presence of blocking peptide.
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Search PubMed (MEDLINE) for references to Bid
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Bid antibody - more information
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Customer reviews (feedback) regarding Bid antibody |
Customer FAQs regarding Bid antibody |
Protocols for Bid antibody |
Price and availability of products related to Bid antibody |
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Bid antibody - related products:
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Bid antibody - other tools:
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Contact Abcam with a Technical Enquiry about Bid antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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