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Products:Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
Overview
Product name
Anti-Bid antibody
See all Bid products (16) ...
Description
Rabbit polyclonal to Bid
Tested applications
WB, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic non-phosphopeptide derived from human Bid around the phosphorylation site of serine 78 (S-E-SP-Q-E).
Positive control
Jurkat cell. Brain tissue.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
Applications
Show applications key
Our Abpromise guarantee covers the use of ab63541 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 26 kDa (predicted molecular weight: 22 kDa).
ELISA
ELISA: 1/10000ELISA: 1/10000
IHC-P: 1/50 - 1/100.
Target
Function
The major proteolytic product p15 BID allows the release of cytochrome c (By similarity). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.
Tissue specificity
Isoform 2 and isoform 3 are expressed in spleen, bone marrow, cerebral and cerebellar cortex. Isoform 2 is expressed in spleen, pancreas and placenta (at protein level). Isoform 3 is expressed in lung, pancreas and spleen (at protein level). Isoform 4 is expressed in lung and pancreas (at protein level).
Domain
Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
Post-translational
modifications
TNF-alpha induces a caspase-mediated cleavage of p22 BID into a major p15 and minor p13 and p11 products.
Phosphorylated upon DNA damage, probably by ATM or ATR.
p15 BID is ubiquitinated by ITCH; ubiquitination results in proteasome-dependent degradation.
Cellular localization
Cytoplasm; Cytoplasm. Mitochondrion membrane. When uncleaved, it is predominantly cytoplasmic; Mitochondrion membrane. A significant proportion of isoform 2 localizes to mitochondria, it may be cleaved constitutively; Mitochondrion membrane. Associated with the mitochondrial membrane and Mitochondrion membrane. Translocates to mitochondria as an integral membrane protein.
Target information above from: UniProt accessionP55957
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Apoptic death agonist antibody
- Apoptotic death agonist antibody
- Apoptotic death agonist BID antibody
see all
Database links
- Entrez Gene: 637 Human
- Entrez Gene: 12122 Mouse
- Entrez Gene: 64625 Rat
- Omim: 601997 Human
- SwissProt: P55957 Human
- SwissProt: P70444 Mouse
- SwissProt: Q9JK60 Rat
- SwissProt: Q9JLT6 Rat
see all
Anti-Bid antibody images:
Western blot - Bid antibody (ab63541)
All lanes : Anti-Bid antibody (ab63541) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells (5ug) treated
with H2O2 (100uM, 30mins)
Lane 2 : Extracts from Jurkat cells (5ug) treated with etoposide (25uM, 60 mins)
Lane 3 : Extracts from Jurkat cells (5ug) treated
with H2O2 (100uM, 30mins) and etoposide (25uM, 60 mins) with immunizing non-phosphopeptide at 5 µg
Predicted band size : 22 kDa
Observed band size : 26 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Bid antibody (ab63541)
Ab63541 (1:50) staining human Bid in human brain tissue by immunohistochemistry using formalin fixed, paraffin embedded tissue.
The left hand panel is without the presence of blocking peptide, whereas the right hand panel is in the presence of blocking peptide.
References for Anti-Bid antibody (ab63541)
ab63541 has not yet been referenced specifically in any publications.
Publishing research using ab63541? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"