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Products:Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
Overview
Product name
Anti-Bid (phospho S65) antibody
See all Bid products (16) ...
Description
Rabbit polyclonal to Bid (phospho S65)
Tested applications
WB, IHC-P, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic phosphopeptide (Human) corresponding to amino acid residues surrounding serine 65, conjugated to KLH
Positive control
Jurkat cell lysate Human hepatocarcinoma tissue
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with the control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
Applications
Show applications key
Our Abpromise guarantee covers the use of ab59975 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/100 - 1/500.Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
IHC-P: 1/50 - 1/100.
ELISA
ELISA: 1/1000ELISA: 1/1000
Target
Function
The major proteolytic product p15 BID allows the release of cytochrome c (By similarity). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.
Tissue specificity
Isoform 2 and isoform 3 are expressed in spleen, bone marrow, cerebral and cerebellar cortex. Isoform 2 is expressed in spleen, pancreas and placenta (at protein level). Isoform 3 is expressed in lung, pancreas and spleen (at protein level). Isoform 4 is expressed in lung and pancreas (at protein level).
Domain
Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
Post-translational
modifications
TNF-alpha induces a caspase-mediated cleavage of p22 BID into a major p15 and minor p13 and p11 products.
Phosphorylated upon DNA damage, probably by ATM or ATR.
p15 BID is ubiquitinated by ITCH; ubiquitination results in proteasome-dependent degradation.
Cellular localization
Cytoplasm; Cytoplasm. Mitochondrion membrane. When uncleaved, it is predominantly cytoplasmic; Mitochondrion membrane. A significant proportion of isoform 2 localizes to mitochondria, it may be cleaved constitutively; Mitochondrion membrane. Associated with the mitochondrial membrane and Mitochondrion membrane. Translocates to mitochondria as an integral membrane protein.
Target information above from: UniProt accessionP55957
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Apoptic death agonist antibody
- Apoptotic death agonist antibody
- Apoptotic death agonist BID antibody
see all
Database links
- Entrez Gene: 637 Human
- Omim: 601997 Human
- SwissProt: P55957 Human
- Unigene: 591054 Human
Anti-Bid (phospho S65) antibody images:
Western blot - Bid (phospho S65) antibody (ab59975)
Anti-Bid (phospho S65) antibody (ab59975) at 1/100 dilution + Jurkat cell lysate
Predicted band size : 22 kDa
Observed band size : 22 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Bid (phospho S65) antibody (ab59975)
Immunohistochemical analysis of formalin-fixed and paraffin-embedded human hepatocarcinoma tissue labelled with ab59975 at 1/50 dilution. Aperoxidase-conjugatted secondary antibody was then used, followed by AEC staining.
References for Anti-Bid (phospho S65) antibody (ab59975)
ab59975 has not yet been referenced specifically in any publications.
Publishing research using ab59975? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"