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Products:Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
Overview
Product name
Anti-Bid (phospho S78) antibody
See all Bid products (16) ...
Description
Rabbit polyclonal to Bid (phospho S78)
Specificity
Detects endogenous levels of Bid only when phosphorylated at serine 78. Recognises human phosphorylated at serine 78, mouse phophorylated at serine 78 and rat phosphorylated at serine 79.
Tested applications
IHC-P, ELISAmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide (Human) from around the phosphorylation site of serine 78 (SESPQE)
Positive control
Human brain tissue
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
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Immunohistochemistry (Paraffin-embedded sections) - Bid (phospho S78) antibody (ab59174)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab59174 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/50 - 1/100.
ELISA
ELISA: 1/5000ELISA: 1/5000
Target
Function
The major proteolytic product p15 BID allows the release of cytochrome c (By similarity). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.
Tissue specificity
Isoform 2 and isoform 3 are expressed in spleen, bone marrow, cerebral and cerebellar cortex. Isoform 2 is expressed in spleen, pancreas and placenta (at protein level). Isoform 3 is expressed in lung, pancreas and spleen (at protein level). Isoform 4 is expressed in lung and pancreas (at protein level).
Domain
Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
Post-translational
modifications
TNF-alpha induces a caspase-mediated cleavage of p22 BID into a major p15 and minor p13 and p11 products.
Phosphorylated upon DNA damage, probably by ATM or ATR.
p15 BID is ubiquitinated by ITCH; ubiquitination results in proteasome-dependent degradation.
Cellular localization
Cytoplasm; Cytoplasm. Mitochondrion membrane. When uncleaved, it is predominantly cytoplasmic; Mitochondrion membrane. A significant proportion of isoform 2 localizes to mitochondria, it may be cleaved constitutively; Mitochondrion membrane. Associated with the mitochondrial membrane and Mitochondrion membrane. Translocates to mitochondria as an integral membrane protein.
Target information above from: UniProt accessionP55957
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Apoptic death agonist antibody
- Apoptotic death agonist antibody
- Apoptotic death agonist BID antibody
see all
Database links
- Entrez Gene: 637 Human
- Entrez Gene: 12122 Mouse
- Entrez Gene: 64625 Rat
- Omim: 601997 Human
- SwissProt: P55957 Human
- SwissProt: P70444 Mouse
- SwissProt: Q9JK60 Rat
- SwissProt: Q9JLT6 Rat
see all
Anti-Bid (phospho S78) antibody images:
Immunohistochemistry (Paraffin-embedded sections) - Bid (phospho S78) antibody (ab59174)
Immunohistochemical analysis of paraffin embedded human brain tissue using ab59174 at 1/50-1/100 dilution. Samples treated with (+) or without (-) phospho peptide.
References for Anti-Bid (phospho S78) antibody (ab59174)
ab59174 has not yet been referenced specifically in any publications.
Publishing research using ab59174? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"