Bid (phospho S78) antibody (ab59174)

Product Name 

Bid (phospho S78) antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Bid (phospho S78)

Immunogen 

Synthetic peptide (Human) from around the phosphorylation site of serine 78 (SES^PQE)

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

Detects endogenous levels of Bid only when phosphorylated at serine 78.
Recognises human phosphorylated at serine 78, mouse phophorylated at serine 78 and rat phosphorylated at serine 79.

Tested applications 
(see key)

ELISA, IHC-P

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: 1/5000.
IHC-P: 1/50 - 1/100.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human brain tissue

Cellular localization 

Isoform 1 and isoform 3: Cytoplasm.
Isoform 2: Mitochondrial membrane.

Research areas 

Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family

Relevance 

Bid, a BH3 domain containing proapoptotic Bcl2 family member, is localized in the cytosolic fraction of cells as an inactive precursor. Its active form is generated upon proteolytic cleavage by caspase 8 in the Fas signaling pathway. Cleaved Bid translocates to mitochondria and releases its potent proapoptotic activity, which in turn induces cytochrome c release and mitochondrial damage. The cytochrome c releasing activity of Bid was antagonized by Bcl2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In animal model studies, Bid deficient mice are found resistant to the lethal effects of death factor signals relayed through Fas.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at -20°C. Stable for 12 months at -20°C

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