Recombinant Anti-Biglycan antibody [EPR2959] (ab109369)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2959] to Biglycan
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Biglycan antibody [EPR2959]
See all Biglycan primary antibodies -
Description
Rabbit monoclonal [EPR2959] to Biglycan -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Rat cartilage, C2C12, Human skeletal muscle and Mouse muscle lysates; L6 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2959 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109369 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
|
Notes |
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Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 42 kDa. |
Target
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Function
May be involved in collagen fiber assembly. -
Tissue specificity
Found in several connective tissues, especially in articular cartilages. -
Sequence similarities
Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.
Contains 12 LRR (leucine-rich) repeats. -
Post-translational
modificationsThe two attached glycosaminoglycan chains can be either chondroitin sulfate or dermatan sulfate. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 633 Human
- Entrez Gene: 12111 Mouse
- Entrez Gene: 25181 Rat
- Omim: 301870 Human
- SwissProt: P21810 Human
- SwissProt: P28653 Mouse
- SwissProt: P47853 Rat
- Unigene: 821 Human
see all -
Alternative names
- BGN antibody
- Biglycan antibody
- Biglycan proteoglycan antibody
see all
Images
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Intracellular flow cytometric analysis of permeabilized L6 cells using ab109369 at 1/100 dilution (red) or a rabbit IgG (negative) (green).
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All lanes : Anti-Biglycan antibody [EPR2959] (ab109369) at 1/1000 dilution
Lane 1 : Rat cartilage lysate
Lane 2 : L6 lysate
Lane 3 : C2C12 lysate
Lane 4 : Human skeletal muscle lysate
Lane 5 : Mouse muscle lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 42 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab109369 has been referenced in 2 publications.
- Liu S et al. Tyrosine kinase LYN is an oncotarget in human cervical cancer: A quantitative proteomic based study. Oncotarget 7:75468-75481 (2016). WB ; Human . PubMed: 27690342
- Tan HT et al. Unravelling the proteome of degenerative human mitral valves. Proteomics 15:2934-44 (2015). WB ; Human . PubMed: 25914152