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Product name
Anti-Bile Acid Receptor NR1H4 antibody
See all Bile Acid Receptor NR1H4 products (7) ...
Description
Rabbit polyclonal to Bile Acid Receptor NR1H4
Tested applications
ICC/IF, WB, ELISA, IHC-P, IPmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Chicken, Hamster, Cow, Dog
Immunogen
Synthetic peptide, corresponding to amino acids 194-243 of Human Bile Acid Receptor NR1H4.
.
Positive control
WB: Fetal lung lysate. IHC-P: Human kidney tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS.
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Energy transfer pathways >> Energy Metabolism
Signal Transduction >> Metabolism >> Lipid metabolism
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Orphan Nuclear Receptors
Signal Transduction >> Metabolism >> Energy Metabolism
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28676 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution.
WB: Use a concentration of 0.5 µg/ml. Predicted molecular weight: 54 kDa.Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA
ELISA: 1/312500.ELISA: 1/312500.
IHC-P: Use a concentration of 4 - 8 µg/ml. Heat mediated antigen retrieval using citrate buffer is recommended.
IP: Use at an assay dependent concentration. PubMed: 21504366
Target
Function
Ligand-activated transcription factor. Receptor for bile acids such as chenodeoxycholic acid, lithocholic acid and deoxycholic acid. Represses the transcription of the cholesterol 7-alpha-hydroxylase gene (CYP7A1) through the induction of NR0B2 or FGF19 expression, via two distinct mechanisms. Activates the intestinal bile acid-binding protein (IBABP). Activates the transcription of bile salt export pump ABCB11 by directly recruiting histone methyltransferase CARM1 to this locus.
Sequence similarities
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ96RI1
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BAR antibody
- Bile acid receptor antibody
- Farnesoid X activated receptor antibody
see all
Database links
- Entrez Gene: 373902 Chicken
- Entrez Gene: 9971 Human
- Entrez Gene: 20186 Mouse
- Entrez Gene: 60351 Rat
- Omim: 603826 Human
- SwissProt: Q3SZL0 Cow
- SwissProt: Q96RI1 Human
- SwissProt: Q3V1T8 Mouse
see all
Anti-Bile Acid Receptor NR1H4 antibody images:
Western blot - Bile Acid Receptor NR1H4 antibody (ab28676)
Anti-Bile Acid Receptor NR1H4 antibody (ab28676) at 0.5 µg/ml + Fetal Lung Lysate
Secondary
Rabbit Anti HR1H4 Antibody at 1/50000 dilution
Predicted band size : 54 kDa
Immunohistochemistry (Paraffin-embedded sections) - Bile Acid Receptor NR1H4 antibody (ab28676)
Ab28676 at a concentration of 4.0-8.0 µg/ml, staining HR1H4 in paraffin embedded human kidney epithelial cells of collecting tubule by Immunohistochemistry. Arrows point to positively labelled cells. Magnification: 400x.
References for Anti-Bile Acid Receptor NR1H4 antibody (ab28676)
This product has been referenced in:
- Mencarelli A et al. Probiotics modulate intestinal expression of nuclear receptor and provide counter-regulatory signals to inflammation-driven adipose tissue activation. PLoS One 6:e22978 (2011). WB; Human.Read more (PubMed: 21829567) »
- Kay HY et al. Nrf2 Inhibits LXRa-Dependent Hepatic Lipogenesis by Competing with FXR for Acetylase Binding. Antioxid Redox Signal : (2011). WB, IP; Human.Read more (PubMed: 21504366) »
See all 4 publications for this product
Publishing research using ab28676? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"