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Anti-Bim antibody [Y36] (ab32158) at 1/500 dilution + A431 cell lysate
Predicted band size : 22 kDa
Observed band size : 22 kDa
Immunohistochemical analysis of paraffin-embedded breast carcinoma using ab32158 at 1/100 dilution.
Anti-Bim antibody [Y36] (ab32158) at 1/500 dilution + Whole cell lysates prepared from human jurkat cells at 200000 cells
Secondary
HRP conjugated Donkey polyclonal to rabbit IgG at 1/2000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 22 kDa
Observed band size : 22 kDa
Exposure time : 30 seconds
Primary diluted in PBS (5% BSA + 0.1% tween20) and incubated with sample for 1 hour and 30 minutes at 20°C.
The image is a courtesy of an anonymous abreview.
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