![Western blot - Bim antibody [Y36] (ab32158) image](#ab32158wb2 copy.jpg)
![Immunohistochemistry (Paraffin-embedded sections) - Bim antibody [Y36] (ab32158) image](#ab32158ihc.jpg)
![Western blot - Bim antibody [Y36] (ab32158) image](#Bim-Primary-antibodies-ab32158-2.jpg)
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Products:Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins
Overview
Product name
Anti-Bim antibody [Y36]
See all Bim products (11) ...
Description
Rabbit monoclonal [Y36] to Bim
Specificity
The antibody can recognize all isoforms of Bim. The antibody does not cross-react with other Bcl-2 family members.
Tested applications
WB, IHC-P, ICC, IPmore details
Cross reactivity
Reacts with
Human
Does not react with
Mouse, Rat
Immunogen
A synthetic peptide corresponding to residues in the exons 2 of human Bim.
Positive control
A431 cell lysate and breast carcinoma tissue.
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine. pH 7.4
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Monoclonal
Clone number
Y36
Isotype
IgG
Research areas
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Cell survival & death
Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> MAPK Pathway
Immunology >> Innate Immunity >> Macrophage / Inflamm.
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab32158 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
IHC-P
ICC
IP
Application notes
ICC: 1/100.
IHC-P: 1/100.
IP: 1/40.
WB: 1/500. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
Is unsuitable for FACS.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Induces apoptosis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than the isoforms BimEL, BimL and BimS. Isoform Bim-gamma induces apoptosis.
Tissue specificity
Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are ubiquitously expressed with a tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney.
Sequence similarities
Belongs to the Bcl-2 family.
Domain
The BH3 motif is required for Bcl-2 binding and cytotoxicity.
Cellular localization
Mitochondrion and Endomembrane system. Associated with intracytoplasmic membranes.
Target information above from: UniProt accessionO43521
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BCL2 like 11 antibody
- Apoptosis facilitator antibody
- B2L11_HUMAN antibody
see all
Database links
- Entrez Gene: 10018 Human
- Omim: 603827 Human
- SwissProt: O43521 Human
- Unigene: 469658 Human
Anti-Bim antibody [Y36] images:
Western blot - Bim antibody [Y36] (ab32158)
![Western blot - Bim antibody [Y36] (ab32158)](/ps/datasheet/Images/32/ab32158/ab32158wb2 copy.jpg)
Anti-Bim antibody [Y36] (ab32158) at 1/500 dilution + A431 cell lysate
Predicted band size : 22 kDa
Observed band size : 22 kDa
Immunohistochemistry (Paraffin-embedded sections) - Bim antibody [Y36] (ab32158)
![Immunohistochemistry (Paraffin-embedded sections) - Bim antibody [Y36] (ab32158)](/ps/datasheet/Images/32/ab32158/ab32158ihc.jpg)
Immunohistochemical analysis of paraffin-embedded breast carcinoma using ab32158 at 1/100 dilution.
Western blot - Bim antibody [Y36] (ab32158)
![Western blot - Bim antibody [Y36] (ab32158)](/ps/datasheet/images/32/ab32158/Bim-Primary-antibodies-ab32158-2.jpg)
Anti-Bim antibody [Y36] (ab32158) at 1/500 dilution + Whole cell lysates prepared from human jurkat cells at 200000 cells
Secondary
HRP conjugated Donkey polyclonal to rabbit IgG at 1/2000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 22 kDa
Observed band size : 22 kDa
Exposure time : 30 seconds
Primary diluted in PBS (5% BSA + 0.1% tween20) and incubated with sample for 1 hour and 30 minutes at 20°C.
The image is a courtesy of an anonymous abreview.
References for Anti-Bim antibody [Y36] (ab32158)
This product has been referenced in:
- Oussaief Let al. Activation of the lytic program of the Epstein-Barr virus in Burkitt's lymphoma cells leads to a two steps downregulation of expression of the proapoptotic protein BimEL, one of which is EBV-late-gene expression dependent. Virology 387:41-9 (2009). ICC/IF; Human.Read more (PubMed: 19254802) »
See 1 publication for this product
Publishing research using ab32158? Please let us know so that we can cite the reference in this datasheet
![Bim antibody [Y36] for WB in Human (32158)](/ps/datasheet/images/32/ab32158/Bim-Primary-antibodies-ab32158-1.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"