Bim antibody [Y36] (ab32158)

Product Name 

Bim antibody [Y36]

Product type 

Primary antibodies

Description 

Rabbit monoclonal [Y36] to Bim

Immunogen 

A synthetic peptide corresponding to residues in the exons 2 of human Bim.

Reacts with 

Hu

Does not react with 
(species key)

Ms, Rat

Specificity 

The antibody can recognize all isoforms of Bim. The antibody does not cross-react with other Bcl-2 family members.

Tested applications 
(see key)

ICC, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ICC: 1/100.
IHC-P: 1/100.
IP: 1/40.
WB: 1/500. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).

Is unsuitable for FACS.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

A431 cell lysate and breast carcinoma tissue.

Cellular localization 

Intracytoplasmic membranes

Research areas 

Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Cell survival & death
Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> MAPK Pathway
Immunology >> Innate Immunity >> Macrophage / Inflamm.
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins

Relevance 

Bim, Bcl-2 interacting mediator of cell death, is a pro-apoptotic protein belonging to the Bcl2 family of proteins containing a Bcl2 homology domain 3 (BH3). It is proapoptotic and exerts its effects by interacting with prosurvival members of the Bcl2 family like Bcl2, BclxL and Bclw. Bim is sequestered in an inactive conformation through binding to the microtubule-associated dynein motor complex. Certain apoptotic stimuli release Bim from microtubules, allowing inhibitory binding to anti-apoptotic Bcl-2 family members and subsequent iniation of apoptosis.

Database links 

Raised in 

Rabbit

Clonality 

Monoclonal

Clone number 

Y36

Isotype 

IgG

Purity 

IgG fraction

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine. pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

Produced under U.S. Patent No. 5,675,063.

See below for Bim antibody [Y36] images, references, products related to ab32158 and other tools.

Bim antibody [Y36] images:

References for Bim antibody [Y36] (ab32158)

  This product has been used in:

Oussaief L et al. Activation of the lytic program of the Epstein-Barr virus in Burkitt's lymphoma cells leads to a two steps downregulation of expression of the proapoptotic protein BimEL, one of which is EBV-late-gene expression dependent. Virology 387:41-9 (2009). ICC/IF; Human. PubMed: 19254802

If you publish research using ab32158 please let us know so that we can cite the reference on this datasheet.

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