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Read our guarantee »Products:Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins
Anti-Bim antibody [Y36]
See all Bim products (11) ...
Rabbit monoclonal [Y36] to Bim
The antibody can recognize all isoforms of Bim. The antibody does not cross-react with other Bcl-2 family members.
WB, IHC-P, ICC, IPmore details
Reacts with
Human
Does not react with
Mouse, Rat
A synthetic peptide corresponding to residues in the exons 2 of human Bim.
A431 cell lysate and breast carcinoma tissue.
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine. pH 7.4
Concentration information loading...
IgG fraction
Monoclonal
Y36
IgG
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Cell survival & death
Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> MAPK Pathway
Immunology >> Innate Immunity >> Macrophage / Inflamm.
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins
Our Abpromise guarantee covers the use of ab32158 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC: 1/100.
IHC-P: 1/100.
IP: 1/40.
WB: 1/500. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
Is unsuitable for FACS.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Induces apoptosis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than the isoforms BimEL, BimL and BimS. Isoform Bim-gamma induces apoptosis.
Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are ubiquitously expressed with a tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney.
Belongs to the Bcl-2 family.
The BH3 motif is required for Bcl-2 binding and cytotoxicity.
Mitochondrion and Endomembrane system. Associated with intracytoplasmic membranes.
Target information above from: UniProt accessionO43521
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - Bim antibody [Y36] (ab32158)
![Western blot - Bim antibody [Y36] (ab32158)](/ps/datasheet/Images/32/ab32158/ab32158wb2 copy.jpg)
Anti-Bim antibody [Y36] (ab32158) at 1/500 dilution + A431 cell lysate
Predicted band size : 22 kDa
Observed band size : 22 kDa
Immunohistochemistry (Paraffin-embedded sections) - Bim antibody [Y36] (ab32158)
![Immunohistochemistry (Paraffin-embedded sections) - Bim antibody [Y36] (ab32158)](/ps/datasheet/Images/32/ab32158/ab32158ihc.jpg)
Immunohistochemical analysis of paraffin-embedded breast carcinoma using ab32158 at 1/100 dilution.
Western blot - Bim antibody [Y36] (ab32158)
![Western blot - Bim antibody [Y36] (ab32158)](/ps/datasheet/images/32/ab32158/Bim-Primary-antibodies-ab32158-2.jpg)
Anti-Bim antibody [Y36] (ab32158) at 1/500 dilution + Whole cell lysates prepared from human jurkat cells at 200000 cells
Secondary
HRP conjugated Donkey polyclonal to rabbit IgG at 1/2000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 22 kDa
Observed band size : 22 kDa
Exposure time : 30 seconds
Primary diluted in PBS (5% BSA + 0.1% tween20) and incubated with sample for 1 hour and 30 minutes at 20°C.
The image is a courtesy of an anonymous abreview.
This product has been referenced in:
See 1 publication for this product
Publishing research using ab32158? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
Concentration not available for this lot.
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![Western blot - Bim antibody [Y36] (ab32158)](/ps/datasheet/Images/32/ab32158/ab32158wb2 copy.jpg)
Anti-Bim antibody [Y36] (ab32158) at 1/500 dilution + A431 cell lysate
Predicted band size : 22 kDa
Observed band size : 22 kDa
![Immunohistochemistry (Paraffin-embedded sections) - Bim antibody [Y36] (ab32158)](/ps/datasheet/Images/32/ab32158/ab32158ihc.jpg)
Immunohistochemical analysis of paraffin-embedded breast carcinoma using ab32158 at 1/100 dilution.
![Western blot - Bim antibody [Y36] (ab32158)](/ps/datasheet/images/32/ab32158/Bim-Primary-antibodies-ab32158-2.jpg)
Anti-Bim antibody [Y36] (ab32158) at 1/500 dilution + Whole cell lysates prepared from human jurkat cells at 200000 cells
Secondary
HRP conjugated Donkey polyclonal to rabbit IgG at 1/2000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 22 kDa
Observed band size : 22 kDa
Exposure time : 30 seconds
Primary diluted in PBS (5% BSA + 0.1% tween20) and incubated with sample for 1 hour and 30 minutes at 20°C.
The image is a courtesy of an anonymous abreview.
1
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