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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab7888? Please let us know so that we can cite the reference in this datasheet
ab7888 has been referenced in 1 publications.
- Amin AR et al. Synergistic growth inhibition of squamous cell carcinoma of the head and neck by erlotinib and epigallocatechin-3-gallate: the role of p53-dependent inhibition of nuclear factor-kappaB. Cancer Prev Res (Phila) 2:538-45 (2009). WB; Human. PubMed: 19470788
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - Bim antibody (ab7888)
All lanes : Anti-Bim antibody (ab7888) at 1 µg/ml
Lane 1 : K562 whole cell lysate
Lane 2 : A549 whole cell lysate
Predicted band size : 23 kDa
Observed band size : 23 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Bim antibody (ab7888)
ab7888 at 20µg/ml staining Bim in human cancer cells by IHC-P
Immunocytochemistry/ Immunofluorescence - Anti-Bim antibody (ab7888)
ICC/IF image of ab7888 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7888, 5µg/ml) overnight at +4ºC. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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