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Products:Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins
Overview
Product name
Anti-Bim antibody
See all Bim products (11) ...
Description
Rabbit polyclonal to Bim
Tested applications
IHC-P, WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Peptide corresponding to amino acids 22 to 40 of human origin. The sequence is identical to that of mouse and differs from that of rat by one amino acid.
Positive control
Human K562 cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS with 0.02% sodium azide
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Cell survival & death
Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> MAPK Pathway
Immunology >> Innate Immunity >> Macrophage / Inflamm.
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab7888 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 20 µg/ml
WB: 1/1000Predicted molecular weight: 23 kDa.
ICC/IF: Use a concentration of 5 µg/ml
Target
Function
Induces apoptosis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than the isoforms BimEL, BimL and BimS. Isoform Bim-gamma induces apoptosis.
Tissue specificity
Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are ubiquitously expressed with a tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney.
Sequence similarities
Belongs to the Bcl-2 family.
Domain
The BH3 motif is required for Bcl-2 binding and cytotoxicity.
Cellular localization
Mitochondrion and Endomembrane system. Associated with intracytoplasmic membranes.
Target information above from: UniProt accessionO43521
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BCL2 like 11 antibody
- Apoptosis facilitator antibody
- B2L11_HUMAN antibody
see all
Database links
- Entrez Gene: 10018 Human
- Entrez Gene: 12125 Mouse
- Entrez Gene: 64547 Rat
- Omim: 603827 Human
- SwissProt: O43521 Human
- SwissProt: O54918 Mouse
- SwissProt: O88498 Rat
- Unigene: 469658 Human
see all
Anti-Bim antibody images:
Western blot - Bim antibody (ab7888)
All lanes : Anti-Bim antibody (ab7888) at 1 µg/ml
Lane 1 : K562 whole cell lysate
Lane 2 : A549 whole cell lysate
Predicted band size : 23 kDa
Observed band size : 23 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Bim antibody (ab7888)
ab7888 at 20µg/ml staining Bim in human cancer cells by IHC-P
Immunocytochemistry/ Immunofluorescence - Anti-Bim antibody (ab7888)
ICC/IF image of ab7888 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7888, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Bim antibody (ab7888)
This product has been referenced in:
- Amin ARet al. Synergistic growth inhibition of squamous cell carcinoma of the head and neck by erlotinib and epigallocatechin-3-gallate: the role of p53-dependent inhibition of nuclear factor-kappaB. Cancer Prev Res (Phila) 2:538-45 (2009). WB; Human.Read more (PubMed: 19470788) »
See 1 publication for this product
Publishing research using ab7888? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"