Anti-Bim antibody, prediluted (ab15185)

Overview

  • Product nameAnti-Bim antibody, prediluted
    See all Bim primary antibodies
  • Description
    Rabbit polyclonal to Bim, prediluted
  • Tested applicationsIHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide (Human) (N terminal).

  • Positive control
    • HeLa cells, Placenta

Applications

Our Abpromise guarantee covers the use of ab15185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notesIHC-P: 1/1. Perform antigen retrieval before commencing with IHC staining protocol by boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionInduces apoptosis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than the isoforms BimEL, BimL and BimS. Isoform Bim-gamma induces apoptosis.
    • Tissue specificityIsoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are ubiquitously expressed with a tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney.
    • Sequence similaritiesBelongs to the Bcl-2 family.
    • DomainThe BH3 motif is required for Bcl-2 binding and cytotoxicity.
    • Cellular localizationMitochondrion and Endomembrane system. Associated with intracytoplasmic membranes.
    • Information by UniProt
    • Database links
    • Alternative names
      • BCL2 like 11 antibody
      • Apoptosis facilitator antibody
      • B2L11_HUMAN antibody
      • BAM antibody
      • Bcl 2 interacting mediator of cell death antibody
      • Bcl 2 interacting protein Bim antibody
      • Bcl 2 like protein 11 antibody
      • Bcl 2 related ovarian death agonist antibody
      • Bcl 2 related ovarian death agonist antibody
      • Bcl-2-like protein 11 antibody
      • BCL2 interacting mediator of cell death antibody
      • BCL2 interacting protein Bim antibody
      • BCL2 like 11 (apoptosis facilitator) antibody
      • BCL2 like 11 antibody
      • BCL2 like 11 apoptosis facilitator antibody
      • BCL2 like protein 11 antibody
      • BCL2 related ovarian death agonist antibody
      • Bcl2-interacting mediator of cell death antibody
      • Bcl2-L-11 antibody
      • BCL2L11 antibody
      • BIM alpha3 antibody
      • BIM alpha4 antibody
      • BIM alpha5 antibody
      • BIM alpha6 antibody
      • BIM antibody
      • BIM beta6 antibody
      • BIM beta7 antibody
      • BimEL antibody
      • BimL antibody
      • BOD antibody
      see all

    References for Anti-Bim antibody, prediluted (ab15185)

    ab15185 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (pancreas)
    Specification pancreas
    Fixative Paraformaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mm citric
    Permeabilization No
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
    Username

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    Verified customer

    Submitted Sep 12 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"