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Anti-Bim antibody, prediluted (ab15185)

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Overview

  • Product nameAnti-Bim antibody, predilutedSee all Bim primary antibodies ...
  • Description
    Rabbit polyclonal to Bim, prediluted
  • Tested applicationsIHC-P more details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide (Human) (N terminal).

  • Positive controlHeLa cells, Placenta

Applications

Our Abpromise guarantee covers the use of ab15185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P
  • Application notesIHC-P: 1/1. Perform antigen retrieval before commencing with IHC staining protocol by boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionInduces apoptosis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than the isoforms BimEL, BimL and BimS. Isoform Bim-gamma induces apoptosis.
    • Tissue specificityIsoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are ubiquitously expressed with a tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney.
    • Sequence similaritiesBelongs to the Bcl-2 family.
    • DomainThe BH3 motif is required for Bcl-2 binding and cytotoxicity.
    • Cellular localizationMitochondrion and Endomembrane system. Associated with intracytoplasmic membranes.

      • Target information above from: UniProt accession O43521 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      see all
    • Alternative names
        BCL2 like 11 antibodyApoptosis facilitator antibodyB2L11_HUMAN antibody
        BAM antibodyBcl 2 interacting mediator of cell death antibodyBcl 2 interacting protein Bim antibodyBcl 2 like protein 11 antibodyBcl 2 related ovarian death agonis antibodyBcl 2 related ovarian death agonist antibodyBcl-2-like protein 11 antibodyBCL2 interacting mediator of cell death antibodyBCL2 interacting protein Bim antibodyBCL2 like 11 (apoptosis facilitator) antibodyBCL2 like 11 antibodyBCL2 like 11 apoptosis facilitator antibodyBCL2 like protein 11 antibodyBCL2 related ovarian death agonist antibodyBcl2-interacting mediator of cell death antibodyBcl2-L-11 antibodyBCL2L11 antibodyBIM alpha3 antibodyBIM alpha4 antibodyBIM alpha5 antibodyBIM alpha6 antibodyBIM antibodyBIM beta6 antibodyBIM beta7 antibodyBimEL antibodyBimL antibodyBOD antibody
      see all

    References for Anti-Bim antibody, prediluted (ab15185)

    ab15185 has not yet been referenced specifically in any publications.

    Publishing research using ab15185 ? Please let us know so that we can cite the reference in this datasheet

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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Bim antibody, prediluted

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    Abreviews
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (pancreas)
    Specification pancreas
    Fixative Paraformaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mm citric
    Permeabilization No
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
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    Submitted Sep 12 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"