Anti-Bim antibody, prediluted (ab15185)

Overview

  • Product name
    Anti-Bim antibody, prediluted
    See all Bim primary antibodies
  • Description
    Rabbit polyclonal to Bim, prediluted
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide (Human) (N terminal).

  • Positive control
    • HeLa cells, Placenta

Applications

Our Abpromise guarantee covers the use of ab15185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notes
    IHC-P: 1/1. Perform antigen retrieval before commencing with IHC staining protocol by boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Induces apoptosis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than the isoforms BimEL, BimL and BimS. Isoform Bim-gamma induces apoptosis.
    • Tissue specificity
      Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are ubiquitously expressed with a tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney.
    • Sequence similarities
      Belongs to the Bcl-2 family.
    • Domain
      The BH3 motif is required for Bcl-2 binding and cytotoxicity.
    • Cellular localization
      Mitochondrion and Endomembrane system. Associated with intracytoplasmic membranes.
    • Information by UniProt
    • Database links
    • Alternative names
      • BCL2 like 11 antibody
      • B2L11_HUMAN antibody
      • BAM antibody
      • Bcl 2 interacting protein Bim antibody
      • Bcl 2 related ovarian death agonist antibody
      • Bcl-2-like protein 11 antibody
      • BCL2 interacting mediator of cell death antibody
      • BCL2 like 11 (apoptosis facilitator) antibody
      • BCL2 like protein 11 antibody
      • Bcl2-interacting mediator of cell death antibody
      • Bcl2-L-11 antibody
      • Bcl2l11 antibody
      • BIM alpha6 antibody
      • BIM antibody
      • BIM beta6 antibody
      • BIM beta7 antibody
      • BimEL antibody
      • BimL antibody
      • BOD antibody
      see all

    References for Anti-Bim antibody, prediluted (ab15185)

    ab15185 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (pancreas)
    Specification
    pancreas
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mm citric
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 12 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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