Recommended dilutions The peptide is used for blocking the activity of anti-Bim. Incubatingthe peptide with equal volume of antibody for 30 min at 37°C usually completely blocks theantibody activity in Western blotting.
Bim, Bcl-2 interacting mediator of cell death, is a pro-apoptotic protein belonging to the Bcl2 family of proteins containing a Bcl2 homology domain 3 (BH3). It is proapoptotic and exerts its effects by interacting with prosurvival members of the Bcl2 family like Bcl2, BclxL and Bclw. Bim is sequestered in an inactive conformation through binding to the microtubule-associated dynein motor complex. Certain apoptotic stimuli release Bim from microtubules, allowing inhibitory binding to anti-apoptotic Bcl-2 family members and subsequent iniation of apoptosis.
Material safety datasheet (MSDS) for this product: Sodium Azide MSDS
Form
Liquid
Concentration
0.200 mg/ml
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Sequence
AERPPQLRPGAPTSLQTEP Amino acids 22 to 40 of human Bim (1)
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Bim peptide is on this datasheet. But please do contact us if you would like any reassurance!
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