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Products:Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins
Overview
Product name
Anti-Bim (phospho S65) antibody
See all Bim products (11) ...
Description
Rabbit polyclonal to Bim (phospho S65)
Tested applications
Cross reactivity
Reacts with
Rat, Human
Predicted to work with
Mouse
Immunogen
Synthetic phosphopeptide derived from the region of rat Bim that contains serine 65 (serine 69 in the human sequence). The sequence is conserved in human and mouse. (unfortunately, the amino acid sequence is considered to be commercially sensitive)
Positive control
Hek293T co-expressing rat Bim and active MEKK1
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, 1% BSA. pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Bim. The final product is generated by affinity chromatography using a Bim-derived peptide that is phosphorylated at serine 65 (serine 69 in the human sequence).
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Cell survival & death
Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> MAPK Pathway
Immunology >> Innate Immunity >> Macrophage / Inflamm.
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins
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Western blot - Bim (phospho S65) antibody (ab17935)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab17935 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000Detects a band of approximately 25 kDa (predicted molecular weight: 17 kDa).
Target
Function
Induces apoptosis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than the isoforms BimEL, BimL and BimS. Isoform Bim-gamma induces apoptosis.
Tissue specificity
Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are ubiquitously expressed with a tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney.
Sequence similarities
Belongs to the Bcl-2 family.
Domain
The BH3 motif is required for Bcl-2 binding and cytotoxicity.
Cellular localization
Mitochondrion and Endomembrane system. Associated with intracytoplasmic membranes.
Target information above from: UniProt accessionO43521
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BCL2 like 11 antibody
- Apoptosis facilitator antibody
- B2L11_HUMAN antibody
see all
Database links
- Entrez Gene: 10018 Human
- Entrez Gene: 12125 Mouse
- Entrez Gene: 64547 Rat
- Omim: 603827 Human
- SwissProt: O43521 Human
- SwissProt: O54918 Mouse
- SwissProt: O88498 Rat
- Unigene: 469658 Human
see all
Anti-Bim (phospho S65) antibody images:
Western blot - Bim (phospho S65) antibody (ab17935)
Predicted band size : 17 kDa
Lysates prepared from Hek293T cells transfected with rat WT Bim alone (1, 4), cotransfected with rat WT Bim and activated MEKK1 (2, 5) or with
mutant S65A Bim and MEKK1 (3, 6), were resolved by SDS-PAGE on a 14% polyacrylamide gel and transferred to PVDF. Membranes were blocked with 3% Milk-TBST buffer for one hour at room temperature, and incubated with Bim Pan antibody (1, 2, 3) or ab17935 (4, 5, 6) for two hours at room temperature in 3% Milk-TBST buffer. After washing, membranes were incubated with a goat F(ab')2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal™ method.
The data show that the signal is detected only in lysates co-expressing rat WT Bim and active MEKK1. This signal is abolished in cells expressing mutant rat Bim S65A and MEKK1, verifying that the signal is site-phosphospecific.
References for Anti-Bim (phospho S65) antibody (ab17935)
ab17935 has not yet been referenced specifically in any publications.
Publishing research using ab17935? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"