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Read our guarantee »Overview
Product name
Biogenesis Western Blot Cocktail
Cross reactivity
Reacts with
Mouse, Rat, Cow, Human
Product overview
The MitoBiogenesis™ western blotting cocktail (ab123545) is designed to study the regulation of mitochondrial biogenesis and cellular stress in response to environmental stimuli. It also can be used to monitor drug-induced effects on mitochondrial biogenesis. The two main components of this cocktail target two proteins, which are each subunits of a different oxidative phosphorylation enzyme complex, one subunit I of Complex IV (COX-I), which is mtDNA-encoded, and the 70kDa subunit of Complex II (SDH-A), which is nDNA-encoded. Complex IV includes several proteins which are encoded in the mitochondrion, while the proteins of Complex II are entirely encoded in the nucleus. An anti-Beta actin antibody is used as loading control.
Cocktail Antibodies:
Mouse CII-70 (SDHA) [2E3GC12FB2AE2] monoclonal:
Amount: 25 µg
Working concentration: 0.5 µg/ml
Mouse CIV-1 (MT-CO1) [1D6E1A8] monoclonal:
Amount: 50 µg
Working concentration: 1 µg/ml
Mouse Beta actin [mAbcam 8224] monoclonal:
Amount: 25 µg
Working concentration: 0.5 µg/ml
Notes
Antibody Purity: Near homogeneity as judged by SDS-PAGE. The antibodies were produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Tested applications
Properties
Storage instructions
Store at +4°C.
| Components | 100 µg |
|---|---|
| Anitbody Cocktail | 1 x 200µl |
Database links
- SwissProt: P23219 Human
- SwissProt: P31040 Human
- SwissProt: P60709 Human
Applications
Show applications key
Our Abpromise guarantee covers the use of ab123545 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent concentration. (The antibody cocktail should be diluted 250 X to a final working concentration of 2µg/mL for western blotting.)
Biogenesis Western Blot Cocktail images:
Western blot - Biogenesis Western Blot Cocktail (ab123545)
All lanes : Biogenesis Western Blot Cocktail (ab123545) at 1/250 dilution
Lane 1 : Human HepG2 cell lysate (vehicle treated)
Lane 2 : Human HepG2 cell lysate (Chloramphenicol treated)
Lane 3 : Human HepG2 cell lysate (DDC treated)
Lane 4 : Human 143B WT cell lysate
Lane 5 : Human 143B p0 cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
GAM-HRP at 1/3000 dilution
Western blot - Biogenesis Western Blot Cocktail (ab123545)
All lanes : Biogenesis Western Blot Cocktail (ab123545) at 1/250 dilution
Lane 1 : Human HepG2 cell lysate
Lane 2 : Human Hela cell lysate
Lane 3 : Human HDFn cell lysate
Lane 4 : Human SH-SY5Y cell lysate
Lane 5 : Rat H9C2 cell lysate
Lane 6 : Rat H4IIE cell lysate
Lane 7 : Mouse 3T3 cell lysate
Lane 8 : Mouse MEF cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
GAM-HRP at 1/3000 dilution
References for Biogenesis Western Blot Cocktail (ab123545)
ab123545 has not yet been referenced specifically in any publications.
Publishing research using ab123545? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"