Anti-Biotin antibody [39-15D9] (ab3780)
Key features and details
- Mouse monoclonal [39-15D9] to Biotin
- Suitable for: ELISA, WB
- Reacts with: Species independent
- Isotype: IgG2b
Overview
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Product name
Anti-Biotin antibody [39-15D9]
See all Biotin primary antibodies -
Description
Mouse monoclonal [39-15D9] to Biotin -
Host species
Mouse -
Specificity
This antibody reacts with both free biotin and biotin bound to a carrier protein. There is no cross reaction with the free carrier protein (determined by ELISA). -
Tested applications
Suitable for: ELISA, WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to Biotin conjugated to keyhole limpet haemocyanin.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Concentration information loading...
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Purity
Purified IgA -
Clonality
Monoclonal -
Clone number
39-15D9 -
Myeloma
unknown -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3780 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ELISA |
1/5000 - 1/10000.
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|
WB |
1/1000 - 1/5000.
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Notes |
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ELISA
1/5000 - 1/10000. |
WB
1/1000 - 1/5000. |
Target
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Relevance
Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da. -
Alternative names
- Vitamin B4 antibody
Datasheets and documents
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Datasheet download
References (2)
ab3780 has been referenced in 2 publications.
- Potluri LP et al. ZipA is required for FtsZ-dependent preseptal peptidoglycan synthesis prior to invagination during cell division. J Bacteriol 194:5334-42 (2012). PubMed: 22843850
- Ballard PL et al. Regulated gene expression in cultured type II cells of adult human lung. Am J Physiol Lung Cell Mol Physiol 299:L36-50 (2010). PubMed: 20382749