Overview

  • Product nameAnti-Biotin antibodySee all Biotin primary antibodies ...
  • Description
    Sheep polyclonal to Biotin
  • SpecificityThis antibody reacts with KLH-Biotin and BSA-Biotin in ELISA and Western blot. There is no reaction with free KLH or BSA.
  • Tested applicationsELISA, ICC, IHC-Fr, WB more details
  • Immunogen

    Full length native Biotin (purified) conjugated to KLH.

Properties

Applications

Our Abpromise guarantee covers the use of ab1228 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA
ICC
IHC-Fr
WB
  • Application notesELISA: Coating: 1/100 – 1/1000.
    Primary: 1/1000 – 1/30000.
    ICC: 1/200 - 1/2000.
    IHC-Fr: 1/200 - 1/2000.
    WB: 1/1000 - 1/30000.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceBiotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
    • Alternative names
      • Vitamin B4 antibody

    References for Anti-Biotin antibody (ab1228)

    This product has been referenced in:
    • Rennolds J  et al. Cadmium regulates the expression of the CFTR chloride channel in human airway epithelial cells. Toxicol Sci 116:349-58 (2010). Read more (PubMed: 20363832) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"