Biotinylated Goat anti-Mouse IgG (H+L) (Ready to Use) (ab64255)
Overview
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Product name
Biotinylated Goat anti-Mouse IgG (H+L) (Ready to Use)
See all IgG reagents -
Tested applications
Suitable for: Sandwich ELISA, IHC-Pmore details -
General notes
STAINING PROTOCOL:- Deparaffinize and rehydrate formalin-fixed paraffin-embedded tissue section.
- Add enough drops of an approriate Hydrogen Peroxide Block to cover the sections. Incubate for 10 minutes. Wash 2 times in buffer.
- Perform appropriate pretreatment if required. Wash 3 times in buffer.
- Apply an appropriate Protein Block and incubate for 5 minutes at room temperature to block nonspecific background staining. Wash 1 time in buffer.
- Apply primary antibody and incubate according to manufacturer's protocol.
- Wash 4 times in buffer. Apply Biotinylated Goat anti Mouse IgG (H+L) solution and incubate for 10 minutes at room temperature. Wash 4 times in buffer.
- Apply enzyme labeled streptavidin and incubate according to manufacturer’s protocol.
- Rinse 4 times in buffer. Place slide in appropriate chromogenic substrate and incubate until desired reaction is achieved. Rinse 4 times in buffer.
- Add enough drops of Hematoxylin to cover the section. Incubate for 1 minute.
- Rinse 7-8 times in tap water. Add Mounting Medium to cover the section.
This reagent is intended to be used in a labeled streptavidin-biotin immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme-labeled streptavidin, and substrate-chromogen.
Properties
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Form
Liquid -
Storage instructions
Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.0985% Sodium azide
Constituent: PBS -
Concentration information loading...
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Reagent notes
This reagent is intended to be used in a labeled streptavidin-biotin immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme-labeled streptavidin, and substrate-chromogen. -
Research areas
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Cellular localization
Secreted -
Alternative names
- Ig gamma 1 chain C region
- Ig gamma 2 chain C region
- Ig gamma 3 chain C region
see all -
Cellular localization
Secreted
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab64255 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Sandwich ELISA |
Use at an assay dependent concentration. PubMed: 23754944
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IHC-P |
Use at an assay dependent dilution.
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Notes |
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Sandwich ELISA
Use at an assay dependent concentration. PubMed: 23754944 |
IHC-P
Use at an assay dependent dilution. |
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (23)
ab64255 has been referenced in 23 publications.
- Agas A et al. Hemolytic iron regulation in traumatic brain injury and alcohol use. Alcohol 109:1-12 (2023). PubMed: 36690222
- Cheon SB & Kim WH Upregulation of SLUG expression in canine mammary gland tumors and its prognostic significance. BMC Vet Res 19:112 (2023). PubMed: 37553661
- Powell JE et al. Targeted gene silencing in the nervous system with CRISPR-Cas13. Sci Adv 8:eabk2485 (2022). PubMed: 35044815
- Wang J et al. Identification of a novel circ_0001946/miR-1290/SOX6 ceRNA network in esophageal squamous cell cancer. Thorac Cancer 13:1299-1310 (2022). PubMed: 35411716
- Delessard M et al. Achievement of complete in vitro spermatogenesis in testicular tissues from prepubertal mice exposed to mono- or polychemotherapy. Sci Rep 12:7407 (2022). PubMed: 35523907