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Biotinylated Goat anti Mouse IgG (H+L) (Ready to Use) (ab64255)

Submit an Abreview Q&A (6)

Overview

  • Product nameBiotinylated Goat anti Mouse IgG (H+L) (Ready to Use)See all IgG reagents ...
  • Tested applicationsIHC-P more details

    • Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferpH: 7.60
    Preservative: 0.0985% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Reagent notes This reagent is intended to be used in a labeled streptavidin-biotin immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme-labeled streptavidin, and substrate-chromogen.
  • Research Areas
  • RelevanceIgG is the prominent type of immunoglobulin existing in the blood. There are four different subcategories of IgG, the major form of immunoglobulin. It coats germs and makes them easier for germ-eating cells to find. It is composed of a heavy chain (with Fc portion and a variable head) and a variable light chain.

    • Applications

    Our Abpromise guarantee covers the use of ab64255 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    IHC-P IHC-P: Use at an assay dependent dilution.

    Biotinylated Goat anti Mouse IgG (H+L) (Ready to Use) images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotinylated Goat anti Mouse IgG (H+L) (Ready to Use) (ab64255)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotinylated Goat anti Mouse IgG (H+L) (Ready to Use) (ab64255)
      Human esophagus tissue section stained with biotinylated Goat anti Mouse IgG (H+L) (ab64255).

    References for Biotinylated Goat anti Mouse IgG (H+L) (Ready to Use) (ab64255)

    ab64255 has not yet been referenced specifically in any publications.

    Publishing research using ab64255 ? Please let us know so that we can cite the reference in this datasheet

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    Submit an Abreview for ab64255 Submit a Question for ab64255

    Displaying 1 - 6 of 6 results for Abreviews and Q&A

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    I want to know the biotin/ antibody protein ratio.

    The ratio is approximately 6-8 biotins per antibody.

    I hope this is helpful. Please contact us again if you have any further questions.

    What is the concentration of your current lot?

    The concentration is always 7ug/mL for this product.
    Please let me know if there is additional information I can provide.

    Inquiry: Can I use Biotinylated Goat anti Mouse IgG (H+L) (Ready to Use) (ab64255) for ELISA? I want to know the concentration of ab64255.

    Thank you for your enquiry.

    Yes you can use ab64255 for ELISA. Please note it comes pre-diluted and is meant to be used neat. Further dilution is possible but if signal intensity is low you could not increase concentration of the secondary.
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    I ordered the product Biotinylated Goat anti Mouse IgG (H+L) (Ready to Use) (ab64255) some time ago

    and I just wanted to ask, if it is intended to use the antibody without diluting it with buffer, because

    I’m missing informatio...

    Read More

    Thank you for contacting us.

    This is a ready to use product. You do not need to dilute it further.

    However if you get high intensity of staining then you may dilute it 1/10, this way you can do more tests and antibody will last longer...

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    I need to develop double IHC analysis on formalin-fixed paraffin-embedded (FFPE) tissue sections of human tumors specimens, and after we have to do laser capture microdissection of positive cells.   I already have two primary antibodies directed against...

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    Thank you for contacting us.

    We do not currently offer double staining IHC kits. Your staining conditions are challenging as, of the two common HRP chromogens, only DAB is alcohol insoluable. While of the common AP chromagens, only BCIP/NBT is ...

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    1) wie rein ist ab64255 (BSA?)? Ich moechte den AK radiomarkieren, biotin auch wichtig. 2) Konzentration/Volumen? "125ml at mg/ml" macht keinen Sinn.

    Vielen Dank für Ihren Anruf. ab64255 wurde über Affinitätschromatographie aus dem Antiserum aufgereinigt; der exakte Reinheitsgrade wurde leider nicht bestimmt. Der Puffer, in dem der Antikörper vorliegt, enthält BSA. Die Konzentration beträgt 7 µg/ml,...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"