Biotinylated Goat anti Mouse IgG (H+L) (Ready to Use) (ab64255)

Product Name 

Biotinylated Goat anti Mouse IgG (H+L) (Ready to Use)

Product type 

IHC reagents

Description 

Biotinylated Goat anti Mouse IgG (H+L) (Ready to Use)

Tested applications 
(see key)

IHC-P

Application notes 
(see key)

Recommended dilutions
IHC-P: Use at an assay dependent dilution.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Kits/ Lysates/ Other >> Tools and Reagents >> IHC Tools/ Reagents
Secondary antibodies >> anti-Rabbit >> IgG >> Biotin
Secondary antibodies >> anti-Mouse >> IgG >> Unconjugated

Relevance 

IgG is the prominent type of immunoglobulin existing in the blood. There are four different subcategories of IgG, the major form of immunoglobulin. It coats germs and makes them easier for germ-eating cells to find. It is composed of a heavy chain (with Fc portion and a variable head) and a variable light chain.


This reagent is intended to be used in a labeled streptavidin-biotin immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme-labeled streptavidin, and substrate-chromogen.

Storage buffer 

Preservative: 0.0985% Sodium Azide
Constituents: PBS, BSA

Material safety datasheet (MSDS) for this product:
Biotinylated Goat anti Mouse IgG (H+L) MSDS

Form 

Liquid

Storage instructions 

Store at +4°C.

Components 

Biotinylated Goat anti Mouse IgG (H+L) 125ml

Constituents:
Biotinylated Goat anti Mouse IgG (H+L) in phosphate buffer with stabilizers that have no mercury.

STAINING PROTOCOL:

1. Deparaffinize and rehydrate formalin-fixed paraffin-embedded tissue section.
   
2. Add enough drops of an approriate Hydrogen Peroxide Block to cover the sections. Incubate for 10 minutes. Wash 2 times in buffer.
   
3. Perform appropriate pretreatment if required. Wash 3 times in buffer.
   
4. Apply an appropriate Protein Block and incubate for 5 minutes at room temperature to block nonspecific background staining. Wash 1 time in buffer.
   
5. Apply primary antibody and incubate according to manufacturer's protocol.
   
6. Wash 4 times in buffer. Apply Biotinylated Goat anti Mouse IgG (H+L) solution and incubate for 10 minutes at room temperature. Wash 4 times in buffer.
   
7. Apply enzyme labeled streptavidin and incubate according to manufacturer’s protocol.
   
8. Rinse 4 times in buffer. Place slide in appropriate chromogenic substrate and incubate until desired reaction is achieved. Rinse 4 times in buffer.
   
9. Add enough drops of Hematoxylin to cover the section. Incubate for 1 minute.
   
10. Rinse 7-8 times in tap water. Add Mounting Medium to cover the section.

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