General notesTissue: Urinary bladder
Location: Tumors with base size 29 x 26 mm at 7 o'clock, and 40 x 31 mm at 8-11 o'clock
Diagnosis: Papillary transitional cell carcinoma
Stage: II T2NxMo
Age: 56 years
Gross findings: Tumors with base size 29 x 26 mm at 7 o'clock, and 40 x 31 mm at 8-11 o'clock
Soluble protein fraction.
Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
Purification notesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.
Lysate notesThis product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction.
Please find below a range of products that relate to ab44611:
Extraction 1: soluble protein fraction
ab44611 Human bladder tumor tissue lysate
ab44609 Human bladder normal tissue lysate (matched)
Extraction 2: insoluble protein fraction
ab44615 Human bladder tumor tissue lysate
ab44614 Human bladder normal tissue lysate (matched)