Bladder (Human) Tissue Lysate - Papillary transitional cell carcinoma (ab44641)

Overview

  • Product nameBladder (Human) Tissue Lysate - Papillary transitional cell carcinoma
    See all Bladder lysates
  • General notesTissue: Urinary bladder Location: Not recorded. Diagnosis: Papillary transitional cell carcinoma. Well differentiated. Stage: Not recorded. Grade: Not recorded. Sex: Male Age: 52 years Gross findings: Tumor size 2 x 2 x 3 cm. Cut surface soft, non-identical, red-white. Hemorrhagic. Insoluble protein fraction.

Properties

  • FormLiquid
  • Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.40
    Constituents: PBS, 0.77% DTT, 0.1% SDS, 30.03% Urea, 15.22% Thiourea, 100% Laemmli buffer
    Note: 20µl of 5X Laemmli buffer were added per 100µl of tissue lysate
  • Concentration information loading...
  • Purification NotesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.
  • Lysate notes This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab44641: Extraction 1: soluble protein fraction ab44638 Human Bladder Tumor Tissue lysate ab46394 Human Bladder Normal Tissue lysate (matched) Extraction 2: insoluble protein fraction ab44641 Human Bladder Tumor Tissue lysate ab46477 Human Bladder Normal Tissue lysate (matched)

    References for Bladder (Human) Tissue Lysate - Papillary transitional cell carcinoma (ab44641)

    ab44641 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"