Bladder (Human) Tissue Lysate - Transitional cell carcinoma (ab44655)

Overview

  • Product nameBladder (Human) Tissue Lysate - Transitional cell carcinoma
    See all Bladder lysates
  • General notesTissue: Urinary bladder Location: Right wall of bladder at 6-9 o'clock Diagnosis: Transitional cell carcinoma, well differentiated Stage: III T3NXM0 Grade: I Sex: Female Age: 45 years Gross findings: Tumor irregular in shape, size 76 x 53mm. Cut section firm, non-identical and pink/red. Invasive and hemorrhagic. Soluble protein fraction

Properties

  • FormLiquid
  • Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.40
    Constituents: PBS, 0.029% EDTA, 0.25% Sodium deoxycholate, 0.018% Sodium orthovanadate, 0.004% Sodium fluoride, 0.1% SDS, 0.017% PMSF, 0.0001% Aprotinin, 0.0001% Leupeptin, 0.0001% Pepstatin A, 100% Laemmli buffer
    Note: 20µl of 5X
  • Concentration information loading...
  • Purification NotesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.
  • Lysate notes This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab44655: Extraction 1: soluble protein fraction ab44655 Human Bladder Tumor Tissue lysate ab44654 Human Bladder Normal Tissue lysate (matched) Extraction 2: insoluble protein fraction ab44657 Human Bladder Tumor Tissue lysate ab44656 Human Bladder Normal Tissue lysate (matched)
  • Research Areas

    References for Bladder (Human) Tissue Lysate - Transitional cell carcinoma (ab44655)

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