Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Cardiovascular >> Blood >> Blood Cell Antigens
Overview
Product name
Anti-Blood Group Lewis x antibody [P12]
See all Blood Group Lewis x products (2) ...
Description
Mouse monoclonal [P12] to Blood Group Lewis x
Specificity
This antibody belongs to the BG-7 group and is specific for Lewis x (Type 2 Chain).
Tested applications
IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Fresh (non-cultured) placenta cell preparation (Human).
Positive control
Human kidney tissue.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, 0.01M PBS
Concentration
Concentration information loading...
Purity
Purified IgM
Primary antibody notes
Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis a, Lewis b, Lewis x, Lewis y, and precursor type 1 chain antigens. This antibody belongs to the BG-7 group and detects the Lewis x (type 2 chain) blood-group antigen, which is expressed in gastrointestinal tumors, and lung cancers. It is found in crypt cells, which represent the proliferative compartment of the colonic mucosa, and strong homogeneous immunostaining is observed in most metastases and invasive cancer cells.
Clonality
Monoclonal
Clone number
P12
Isotype
IgM
Research areas
Cardiovascular >> Blood >> Blood Cell Antigens >> RBC Antigens >> Blood Group Antigens
Cardiovascular >> Blood >> Blood Cell Antigens
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3358 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P
IHC-P: 1/40IHC-P: 1/40
ICC/IF
ICC/IF: 1/200 - 1/400.ICC/IF: 1/200 - 1/400.
Target
Alternative names
- Le(x) antibody
- Lewis x Blood Group antibody
References for Anti-Blood Group Lewis x antibody [P12] (ab3358)
This product has been referenced in:
- Shi CSet al. Lectin-like domain of thrombomodulin binds to its specific ligand Lewis Y antigen and neutralizes lipopolysaccharide-induced inflammatory response. Blood : (2008).Read more (PubMed: 18711002) »
- Nyström Ket al. Virus-induced transcriptional activation of host FUT genes associated with neo-expression of Ley in cytomegalovirus-infected and sialyl-Lex in varicella-zoster virus-infected diploid human cells. Glycobiology 17:355-66 (2007).Read more (PubMed: 17202152) »
See all 2 publications for this product
Publishing research using ab3358? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"