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Products:Cardiovascular >> Blood >> Blood Cell Antigens
Overview
Product name
Anti-Blood Group Lewis y antibody [F3]
Description
Mouse monoclonal [F3] to Blood Group Lewis y
Specificity
This antibody belongs to the BG-8 group of murine monoclonal antibodies and is specific against the Lewis y (Type 2 Chain) antigen.
Tested applications
Flow Cyt, IHC-P, WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
SK-LU-3 lung cancer cell line (Human).
Positive control
Colon adenocarcinoma (human).
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, 0.01M PBS
Concentration
Concentration information loading...
Purity
Purified IgM
Primary antibody notes
Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis a, Lewis b, Lewis x, Lewis y, and precursor type 1 chain antigens. Lewis y (type 2 chain) antigen is a difucosylated tetrasaccharide found on the Type 2 blood group oligosaccharides of glycolipids and glycoproteins. It is expressed in large bowel tumors and colorectal carcinomas. It may be useful in the classification of human renal and bladder tumors. The Lewis y antigen has been evaluated as a clinical marker for the diagnosis and prognosis of cholangiocarcinoma, hepatocellular carcinoma and breast cancer.
Clonality
Monoclonal
Clone number
F3
Isotype
IgM
Research areas
Cardiovascular >> Blood >> Blood Cell Antigens >> RBC Antigens >> Blood Group Antigens
Cardiovascular >> Blood >> Blood Cell Antigens
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3359 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use at an assay dependent dilution.
IHC-P
IHC-P: 1/40IHC-P: 1/40
WB: Use at an assay dependent dilution.
ICC/IF: 1/200 - 1/400.(PubMed: 17202152)
Target
Alternative names
- FUT3 antibody
- Lewis Y blood group antibody
References for Anti-Blood Group Lewis y antibody [F3] (ab3359)
This product has been referenced in:
- Yavuz Eet al. Glycomimicry: display of fucosylation on the lipo-oligosaccharide of recombinant Escherichia coli K12. Glycoconj J 28:39-47 (2011). WB.Read more (PubMed: 21286806) »
- Yeh CYet al. C-terminal repeats of Clostridium difficile toxin A induce production of chemokine and adhesion molecules in endothelial cells and promote migration of leukocytes. Infect Immun 76:1170-8 (2008).Read more (PubMed: 18160482) »
See all 6 publications for this product
Publishing research using ab3359? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"