Blooms Syndrome Protein Blm antibody [BFL-103] (ab5409)

Product Name 

Blooms Syndrome Protein Blm antibody [BFL-103]

Product type 

Primary antibodies

Description 

Mouse monoclonal [BFL-103] to Blooms Syndrome Protein Blm

Immunogen 

Recombinant full length protein (Human).

Reacts with 
(species key)

Hu

Tested applications 
(see key)

IHC-Fr, WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
IHC-Fr: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Detects a band of approximately 190 kDa (predicted molecular weight: 160 kDa).

Is unsuitable for IF. This antibody did not appear to work for IF in either HeLa or SK-N-SH cells. Working concentrations of 0.7µg/ml and 3.5µg/ml were tried.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Homologous Recomb.

Relevance 

The Bloom's syndrome (BS) gene, BLM, plays an important role in the maintenance of genomic stability in somatic cells. The BLM protein is a 1417 amino acid peptide with homology to the RecQ helicases, a subfamily of DExH box-containing DNA and RNA helicases. The BLM protein has similarity to 2 other proteins that are members of the subfamily, namely the gene product encoded by RECQL2, also called the Werner syndrome gene (WRN), and the product of the yeast gene SGS1. These proteins may interact with topoisomerases, have 42 to 44% amino acid identity across the conserved helicase motifs, are of similar length and contain highly negatively charged N-terminal regions and highly positively charged C-terminal regions.

The BLM protein is located in the nucleus of normal human cells in the nuclear domain 10 (ND10) or promyelocytic leukemia nuclear (PML) bodies. These structures are punctate deposits of proteins disrupted upon viral infection and in certain human malignancies. BLM was found primarily in ND10 except during S phase, when it colocalized with the Werner syndrome gene product, WRN, in the nucleolus. The BLM protein is likely to be part of a DNA surveillance mechanism operating during S phase - BLM was found to be part of the BASC (BRCA1-associated genome surveillance) complex, which may serve as a sensor of abnormal DNA structures and/or as a regulator of the postreplication repair process.

Bloom syndrome cells show marked genomic instability; in particular, hyperrecombination between sister chromatids and homologous chromosomes - SCE (sister chromatid exchanges). In vitro BLM selectively binds Holliday junctions formed during genetic recombination and acts on recombination intermediates containing a Holliday junction to promote ATP-dependent branch migration. BLM may disrupt potentially recombinogenic molecules that arise at sites of stalled replication forks.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

BFL-103

Myeloma 

NS1

Isotype 

IgG1

Purity 

Protein A purified

Storage buffer 

Preservative: None
Constituents: PBS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

References for Blooms Syndrome Protein Blm antibody [BFL-103] (ab5409)

  This product has been used in: (two most recent references)

Verdun RE & Karlseder J The DNA damage machinery and homologous recombination pathway act consecutively to protect human telomeres. Cell 127:709-20 (2006). PubMed: 17110331

Turley H et al. The distribution and expression of the Bloom's syndrome gene product in normal and neoplastic human cells. Br J Cancer 85:261-5 (2001). PubMed: 11461087

See all 3 publication references for this product.


If you publish research using ab5409 please let us know so that we can cite the reference on this datasheet.

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