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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Homologous Recomb.
Overview
Product name
Anti-Blooms Syndrome Protein Blm antibody [BFL-103]
See all Blooms Syndrome Protein Blm products (8) ...
Description
Mouse monoclonal [BFL-103] to Blooms Syndrome Protein Blm
Tested applications
WB, IHC-Frmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length protein (Human).
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
BFL-103
Myeloma
NS1
Isotype
IgG1
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Homologous Recomb.
Applications
Show applications key
Our Abpromise guarantee covers the use of ab5409 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
IHC-Fr
Application notes
IHC-Fr: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Detects a band of approximately 190 kDa (predicted molecular weight: 160 kDa).
Is unsuitable for IF. This antibody did not appear to work for IF in either HeLa or SK-N-SH cells. Working concentrations of 0.7µg/ml and 3.5µg/ml were tried.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction.
Involvement in disease
Defects in BLM are the cause of Bloom syndrome (BLM) [MIM:210900]. BLM is an autosomal recessive disorder characterized by proportionate pre- and postnatal growth deficiency, sun-sensitive telangiectatic hypo- and hyperpigmented skin, predisposition to malignancy, and chromosomal instability.
Sequence similarities
Belongs to the helicase family. RecQ subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 HRDC domain.
Post-translational
modifications
Phosphorylated in response to DNA damage. Phosphorylation requires the FANCA-FANCC-FANCE-FANCF-FANCG protein complex, as well as the presence of RMI1.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP54132
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- RecQ-like type 2 antibody
- BLM antibody
- BLM_HUMAN antibody
see all
Database links
- Entrez Gene: 641 Human
- Omim: 604610 Human
- SwissProt: P54132 Human
- Unigene: 169348 Human
- Unigene: 725208 Human
References for Anti-Blooms Syndrome Protein Blm antibody [BFL-103] (ab5409)
This product has been referenced in:
- Verdun RE & Karlseder J The DNA damage machinery and homologous recombination pathway act consecutively to protect human telomeres. Cell 127:709-20 (2006).Read more (PubMed: 17110331) »
- Turley Het al. The distribution and expression of the Bloom's syndrome gene product in normal and neoplastic human cells. Br J Cancer 85:261-5 (2001).Read more (PubMed: 11461087) »
See all 3 publications for this product
Publishing research using ab5409? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"