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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Homologous Recomb.
Overview
Product name
Anti-Blooms Syndrome Protein Blm antibody
See all Blooms Syndrome Protein Blm products (8) ...
Description
Rabbit polyclonal to Blooms Syndrome Protein Blm
Tested applications
IF, WB, IP, ICCmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide (Human) conjugated to KLH - which represents a portion of human Bloom Syndrome protein (Blm) encoded within exon 22 (LocusLink ID 641)
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Tris-citrate/phosphate, pH 7-8, 0.1% sodium azide
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Homologous Recomb.
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2179 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IF
IP
Application notes
ICC/IF: Use at a concentration of 5 µg/ml.
IP: Use at a concentration of 1 - 4 µg/ml.
WB: 1/1000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 159 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction.
Involvement in disease
Defects in BLM are the cause of Bloom syndrome (BLM) [MIM:210900]. BLM is an autosomal recessive disorder characterized by proportionate pre- and postnatal growth deficiency, sun-sensitive telangiectatic hypo- and hyperpigmented skin, predisposition to malignancy, and chromosomal instability.
Sequence similarities
Belongs to the helicase family. RecQ subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 HRDC domain.
Post-translational
modifications
Phosphorylated in response to DNA damage. Phosphorylation requires the FANCA-FANCC-FANCE-FANCF-FANCG protein complex, as well as the presence of RMI1.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP54132
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- RecQ-like type 2 antibody
- BLM antibody
- BLM_HUMAN antibody
see all
Database links
- Entrez Gene: 641 Human
- Omim: 604610 Human
- SwissProt: P54132 Human
- Unigene: 169348 Human
- Unigene: 725208 Human
Anti-Blooms Syndrome Protein Blm antibody images:
Immunocytochemistry - Blooms Syndrome Protein Blm antibody (ab2179)
HeLa cells cultured on coverslips were fixed in 4% paraformaldehyde then stained with ab2179 (green) at a concentration of 5µg/ml. The DNA stained with DAPI is shown in red (100x magnification).
This image was submitted as part of a review by Kirk McManus, University of British Columbia
Immunocytochemistry - Blooms Syndrome Protein Blm antibody (ab2179)
HeLa cells were treated with 15µM Bleomycin for 2 hours to generate DNA double stranded breaks (DSBs). Paraformaldehyde-fixed cells were co-immunofluorescently labelled with anti-gamma H2AX (DNA DSB marker) and anti-BLM (ab2179). ab 2179 was used at a concentration of 5µg/ml. Colocalization at the sites of DNA DSBs is identified by the arrows. In the final panel DAPI is pseudo-coloured blue, while anti-gamma H2AX and anti-Blm are green and red respectively.
This image was submitted as part of a review by Kirk McManus, University of British Columbia
References for Anti-Blooms Syndrome Protein Blm antibody (ab2179)
This product has been referenced in:
- Ke Yet al. PICH and BLM limit histone association with anaphase centromeric DNA threads and promote their resolution. EMBO J 30:3309-21 (2011). WB, ICC/IF; Human.Read more (PubMed: 21743438) »
See 1 publication for this product
Publishing research using ab2179? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"