Blooms Syndrome Protein Blm antibody (ab476)

Product Name 

Blooms Syndrome Protein Blm antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Blooms Syndrome Protein Blm

Immunogen 

His-tagged 376 amino acid C-terminal of human blm fusion protein.

Reacts with 

Hu

Predicted to react 
(species key)

Mamm
(Due to sequence homology)

Tested applications 
(see key)

ICC, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ICC: Use at an assay dependent dilution(from PubMed:15498034).
IP: Use at an assay dependent dilution.
WB: 1/2000. Detects a band of approximately 170 kDa (predicted molecular weight: 159 kDa).

We have conflicting reports from customers about whether this antibody works in IF in HeLa or SK-N-SH cells. We would appreciate any customer feedback about this antibody.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

HeLa nuclear extract.

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Homologous Recomb.

Relevance 

The Bloom's syndrome (BS) gene, BLM, plays an important role in the maintenance of genomic stability in somatic cells. The BLM protein is a 1417 amino acid peptide with homology to the RecQ helicases, a subfamily of DExH box-containing DNA and RNA helicases. The BLM protein has similarity to 2 other proteins that are members of the subfamily, namely the gene product encoded by RECQL2, also called the Werner syndrome gene (WRN), and the product of the yeast gene SGS1. These proteins may interact with topoisomerases, have 42 to 44% amino acid identity across the conserved helicase motifs, are of similar length and contain highly negatively charged N-terminal regions and highly positively charged C-terminal regions.

The BLM protein is located in the nucleus of normal human cells in the nuclear domain 10 (ND10) or promyelocytic leukemia nuclear (PML) bodies. These structures are punctate deposits of proteins disrupted upon viral infection and in certain human malignancies. BLM was found primarily in ND10 except during S phase, when it colocalized with the Werner syndrome gene product, WRN, in the nucleolus. The BLM protein is likely to be part of a DNA surveillance mechanism operating during S phase - BLM was found to be part of the BASC (BRCA1-associated genome surveillance) complex, which may serve as a sensor of abnormal DNA structures and/or as a regulator of the postreplication repair process.

Bloom syndrome cells show marked genomic instability; in particular, hyperrecombination between sister chromatids and homologous chromosomes - SCE (sister chromatid exchanges). In vitro BLM selectively binds Holliday junctions formed during genetic recombination and acts on recombination intermediates containing a Holliday junction to promote ATP-dependent branch migration. BLM may disrupt potentially recombinogenic molecules that arise at sites of stalled replication forks.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

n/a

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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References for Blooms Syndrome Protein Blm antibody (ab476)

  This product has been used in: (two most recent references)

So S et al. Enhanced gene targeting efficiency by siRNA that silences the expression of the Bloom syndrome gene in human cells. Genes Cells 11:363-71 (2006). PubMed: 16611240

Raynard S et al. A double Holliday junction dissolvasome comprising BLM, topoisomerase IIIalpha, and BLAP75. J Biol Chem 281:13861-4 (2006). WB; Human. PubMed: 16595695

See all 10 publication references for this product.


If you publish research using ab476 please let us know so that we can cite the reference on this datasheet.

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