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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Homologous Recomb.
Overview
Product name
Anti-Blooms Syndrome Protein Blm antibody
See all Blooms Syndrome Protein Blm products (8) ...
Description
Rabbit polyclonal to Blooms Syndrome Protein Blm
Tested applications
WB, IP, ICCmore details
Cross reactivity
Reacts with
Human
Predicted to work with
all Mammals
Immunogen
His-tagged 376 amino acid C-terminal of human blm fusion protein.
Positive control
HeLa nuclear extract.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Homologous Recomb.
Applications
Show applications key
Our Abpromise guarantee covers the use of ab476 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000Detects a band of approximately 170 kDa (predicted molecular weight: 159 kDa).(We have conflicting reports from customers about whether this antibody works in IF in HeLa or SK-N-SH cells. We would appreciate any customer feedback about this antibody.)
IP: Use at an assay dependent dilution.
ICC: Use at an assay dependent dilution.
Target
Function
Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction.
Involvement in disease
Defects in BLM are the cause of Bloom syndrome (BLM) [MIM:210900]. BLM is an autosomal recessive disorder characterized by proportionate pre- and postnatal growth deficiency, sun-sensitive telangiectatic hypo- and hyperpigmented skin, predisposition to malignancy, and chromosomal instability.
Sequence similarities
Belongs to the helicase family. RecQ subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 HRDC domain.
Post-translational
modifications
Phosphorylated in response to DNA damage. Phosphorylation requires the FANCA-FANCC-FANCE-FANCF-FANCG protein complex, as well as the presence of RMI1.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP54132
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- RecQ-like type 2 antibody
- BLM antibody
- BLM_HUMAN antibody
see all
Database links
- Entrez Gene: 641 Human
- Omim: 604610 Human
- SwissProt: P54132 Human
- Unigene: 169348 Human
- Unigene: 725208 Human
Anti-Blooms Syndrome Protein Blm antibody images:
Western blot - Blooms Syndrome Protein Blm antibody (ab476)
Predicted band size : 159 kDa
Western blot using ab476 against HELA nuclear extract revealing the 160kD Blm protein which, in gels, moves as a 190kD band.
Immunoprecipitation - Blooms Syndrome Protein Blm antibody (ab476)
BLAP75 physically interacts with BLM and Topo IIIa. Purified BLM was incubated with purified Topo IIIa and the reaction mixture was subjected to Immunoprecipitation with ab476. The reaction supernatant (S), wash (W), and eluate (E) were analyzed by SDS-PAGE, GST-BLAP75 or GST alone was incubated with BLM. Protein complexes were captured on glutathione-Sepharose beads, followed by SDS-PAGE.
Image from S Raynard et al, J Biol Chem 281:13861-4 (2006), Fig 2.
References for Anti-Blooms Syndrome Protein Blm antibody (ab476)
This product has been referenced in:
- Laulier Cet al. The relative efficiency of homology-directed repair has distinct effects on proper anaphase chromosome separation. Nucleic Acids Res : (2011). WB; Human.Read more (PubMed: 21459848) »
- Lahkim Bennani-Belhaj Ket al. The Bloom syndrome protein limits the lethality associated with RAD51 deficiency. Mol Cancer Res 8:385-94 (2010). WB, ICC/IF; Human.Read more (PubMed: 20215422) »
See all 13 publications for this product
Publishing research using ab476? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"