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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Homologous Recomb.
Overview
Product name
Anti-Blooms Syndrome Protein Blm antibody
See all Blooms Syndrome Protein Blm products (8) ...
Description
Goat polyclonal to Blooms Syndrome Protein Blm
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Human
Immunogen
Immunogen was a synthetic peptide, which represented a portion of human Bloom Syndrome encoded within exon 2.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: None
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Homologous Recomb.
Applications
Show applications key
Our Abpromise guarantee covers the use of ab5446 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
IP
Application notes
IP: Use at a concentration of 1 µg/ml.
WB: 1/1000 - 1/10000. Detects a band of approximately 190 kDa (predicted molecular weight: 180 kDa).
We have had reports that this antibody did not work in IF in either HeLa or SK-N-SH cells at the concentrations tired (5µg/ml and 1µg/ml). We would appreciate customer feedback about this antibody.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction.
Involvement in disease
Defects in BLM are the cause of Bloom syndrome (BLM) [MIM:210900]. BLM is an autosomal recessive disorder characterized by proportionate pre- and postnatal growth deficiency, sun-sensitive telangiectatic hypo- and hyperpigmented skin, predisposition to malignancy, and chromosomal instability.
Sequence similarities
Belongs to the helicase family. RecQ subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 HRDC domain.
Post-translational
modifications
Phosphorylated in response to DNA damage. Phosphorylation requires the FANCA-FANCC-FANCE-FANCF-FANCG protein complex, as well as the presence of RMI1.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP54132
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- RecQ-like type 2 antibody
- BLM antibody
- BLM_HUMAN antibody
see all
Database links
- Entrez Gene: 641 Human
- Omim: 604610 Human
- SwissProt: P54132 Human
- Unigene: 169348 Human
- Unigene: 725208 Human
References for Anti-Blooms Syndrome Protein Blm antibody (ab5446)
ab5446 has not yet been referenced specifically in any publications.
Publishing research using ab5446? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"